The enzyme that seals 5’ PO4 and 3’ OH polynucleotide ends while creating a recombinant DNA molecule is

1. Alkaline phosphatase

2. DNA ligase

3. DNAse

4. Restriction endonuclease

Part of a plasmid such as pBR322 responsible for controlling the copy number of the linked alien DNA is

1. Ori

2. rop

3. amp^R

4. tet^R

DNA from Agrobacterium tumefaciens can be extracted through use of enzyme

1. Chitinase

2. Cellulase

3. Lysozyme

4. DNAse

Select the incorrect statement.

1. Separation of DNA fragments occurs based on their size in agarose gel.

2. Blue-white selection involves insertional inactivation of β-galactosidase.

3. Treatment with ice-cold calcium can enhance efficiency of transformation in host cells.

4. Extension step during PCR is based on thermolabile nature of Taq polymerase.

All the following are transformation procedures except

1. Microinjection

2. Biolistics

3. Spooling

4. Electroporation

Downstream processing does not involve

1. Biosynthesis

2. Separation

3. Purification

4. Centrifugation

If a foreign gene is inserted into pBR322 by using restriction enzyme BamHI, the selection process, for identifying recombinants requires plating on all except

1. Ampicillin rich medium

2. Tetracycline rich medium

3. X-gal rich medium

4. Nutrient medium without antibiotics

If any protein-encoding gene is expressed in a heterologous host, it is termed as

1. Recombinant protein

2. Single-cell protein

3. Humulin

4. Transposon

Read the following statements

Statement-A : Restriction enzymes are obtained only from eukaryotes.

Statement-B : The first discovered restriction endonuclease was EcoRI.

Choose the correct option.

(1) Both statements are correct

(2) Both statements are incorrect

(3) Statement A is correct but B is incorrect

(4) Statement B is correct but A is incorrect

Genetically engineered plants are generated in laboratory by altering their genetic makeup. This is done by adding one or more genes to a plant’s genome by

(1) Biolistic method or Agrobacterium tumefaciens mediated transformation

(2) DNA injection or heat shock method

(3) Fusing liposomes to intact plant cells

(4) Bacillus thuringiensis mediated transformation