Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so, special shown in it? 
5’___GAATTC___3’ 
3’___CTTAAG__5’

1.  Deletion mutation

2.  Start codon at the 5' end

3.  Palindromic sequence of base pairs

4.  Replication completed

Subtopic:  Tools: Enzymes: II | Restriction Enzymes - Main Enzymes | Tools |
 95%
From NCERT
AIPMT - 2011
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There is a restriction endonuclease called Eco RI. What does the 'co' part in it stand for?
1.  Coelom
2.  Coenzyme
3.  Coli
4.  Colon
Subtopic:  Restriction Enzymes - Main Enzymes |
 90%
From NCERT
AIPMT - 2011
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Agarose extracted from seaweeds finds use in:
1.  tissue culture
2.  PCR
3.  gel electrophoresis
4.  spectrophotometry
Subtopic:  Separation and Isolation of DNA fragments |
 95%
From NCERT
AIPMT - 2011
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Continuous addition of sugars in 'fed-batch' fermentation is done to:

1.  obtain antibiotics

2.  purify enzymes

3.  degrade sewage

4.  produce methane

Subtopic:  Large Scale Production: II | Bioreactors/Downstream Processing | Large Scale Production |
AIPMT - 2011
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Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

1. 5'-CGTTCG-3' 2. 5'-GATATG-3'
3'-ATGGTA-5' 3'-CTACTA-5'
3. 5'-GAATTC-3' 4. 5'-CACGTA-3'
3'-CTTAAG-5' 3'-CTCAGT-5'
Subtopic:  Restriction Enzymes - Main Enzymes |
 94%
From NCERT
AIPMT - 2010
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Restriction endonucleases are enzymes which:

1. make cuts at specific positions within the DNA molecule
2. recognize a specific nucleotide sequence for binding
of DNA ligase
3. restrict the action of the enzyme DNA polymerase
4. remove nucleotides from the ends of the DNA molecule 

Subtopic:  Restriction Enzymes - Main Enzymes |
 92%
From NCERT
AIPMT - 2010
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Stirred-tank bioreactors have been designed for:
1. Addition of preservatives to the product
2. Purification of the product
3. Ensuring anaerobic conditions in the culture vessel
4. Availability of oxygen throughout the process

Subtopic:  Bioreactors/Downstream Processing |
 84%
From NCERT
AIPMT - 2010
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Gel electrophoresis is used for:
1. cutting of DNA into fragments
2. separation of DNA fragments according to their size
3. construction of recombinant DNA by joining with cloning vectors
4. isolation of DNA molecule

Subtopic:  Process of Biotech | Separation and Isolation of DNA fragments |
 90%
From NCERT
AIPMT - 2008
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The linking of the antibiotic resistance gene with the plasmid vector became possible with:

1. DNA ligase

2. endonucleases

3. DNA polymerase

4. exonucleases

Subtopic:  Tools: Enzymes: II | Restriction Enzymes - Main Enzymes | Separation and Isolation of DNA fragments | Tools |
 88%
From NCERT
AIPMT - 2008
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Restriction endonuclease:

1. cuts the DNA molecule randomly

2. cuts the DNA molecule at specific sites

3. restricts the synthesis of DNA inside the nucleus

4. synthesis DNA

Subtopic:  Restriction Enzymes - Main Enzymes |
 91%
From NCERT
AIPMT - 2006
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