Q. No.The figure below is the diagrammatic representation of the E.coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)?
1. Ori-original restriction enzyme
2. Rop-reduced osmotic pressure
3. Hind III, Eco RI-selectable markers
4. AmpR, tetiR-antibiotic resistance genes
1. Ori-original restriction enzyme
Which one of the following is a case of wrong matching?
| 1. | Somatic hybridization- Fusion of two diverse cells |
| 2. | Vector DNA- Site for tRNA synthesis |
| 3. | Micropropagation- In vitro production of plants in large numbers |
| 4. | Callus - Unorganized mass of cells produced in tissue culture |
Which one is a true statement regarding DNA polymerase used in PCR?
1. It is used to ligate introduces DNA in recipient cells
2. It serves as a selectable marker
3. It is isolated from a virus
4. It remains active at high temperatures
For transformation, microparticles coated with DNA to be bombarded with gene gun are made up of:
1. Silver or platinum
2. Platinum or zinc
3. Silicon or platinum
4. Gold or tungsten
5’___GAATTC___3’
3’___CTTAAG__5’
1. Deletion mutation
2. Start codon at the 5' end
3. Palindromic sequence of base pairs
4. Replication completed
1. Coelom
2. Coenzyme
3. Coli
4. Colon
1. tissue culture
2. PCR
3. gel electrophoresis
4. spectrophotometry
1. obtain antibiotics
2. purify enzymes
3. degrade sewage
4. produce methane
Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
| 1. | 5'-CGTTCG-3' | 2. | 5'-GATATG-3' |
| 3'-ATGGTA-5' | 3'-CTACTA-5' | ||
| 3. | 5'-GAATTC-3' | 4. | 5'-CACGTA-3' |
| 3'-CTTAAG-5' | 3'-CTCAGT-5' |
Restriction endonucleases are enzymes which:
| 1. | make cuts at specific positions within the DNA molecule |
| 2. | recognize a specific nucleotide sequence for binding of DNA ligase |
| 3. | restrict the action of the enzyme DNA polymerase |
| 4. | remove nucleotides from the ends of the DNA molecule |
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