Which of the following techniques offer vast potential for manipulation of plants in-vitro to produce new varieties?

1. Hybridisation and selection

2. Tissue culture and somatic hybridisation

3. Conventional breeding and introduction

4. Polyploid breeding and hybridization

Subtopic:  Plant Tissue Culture |
 82%
From NCERT
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Which of the following statements is false?

1. The capacity to generate a whole plant from any cell is called totipotency

2. In tissue culture, the nutrient medium must provide a carbon source such as sucrose, nitrogen source such as urea, vitamins and growth regulators like auxins, cytokinins

3. When a plant is infected with a virus, the meristem (apical and axillary) is free of virus

4. Somatic hybrids are obtained by fusing isolated protoplasts from two different varieties of plants

Subtopic:  Plant Tissue Culture |
 60%
From NCERT
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Recovery of healthy plants from diseased plant is possible by

1. Meristem culture

2. Somatic hybridisation

3. Protoplast fusion

4. Callus culture

Subtopic:  Plant Tissue Culture |
 87%
From NCERT
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In virus-infected plants, the meristematic tissues in both apical and axillary buds are free of virus because

1. the dividing cells are virus resistant
2. meristems have antiviral compounds
3. the cell division of meristems are faster than the rate of viral multiplication
4. viruses cannot multiply within meristem cell(s)

Subtopic:  Plant Tissue Culture |
 65%
From NCERT
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The term ‘totipotency’ refers to the capacity of a

1. cell to generate whole plant

2. bud to generate whole plant

3. seed to germinate

4. cell to enlarge in size

Subtopic:  Plant Tissue Culture |
 95%
From NCERT
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Given below are a few statements regarding somatic hybridization. Choose the correct statements.

I. Protoplasts of different cells of the same plant are fused.
II. Protoplasts from cells of different species can be fused.
III. Treatment of cells with cellulase and pectinase is mandatory.
IV. The hybrid protoplast contains characters of only one parental protoplast.

1. I and III

2. I and II

3. I and IV

4. II and III

Subtopic:  Plant Tissue Culture |
 67%
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An explant is

1. dead plant

2. part of the plant

3. part of the plant used in tissue culture

4. part of the plant that expresses a specific gene

Subtopic:  Plant Tissue Culture |
 89%
From NCERT
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Micro-propagation is

1. propagation of microbes in vitro

2. propagation of plants in vitro

3. propagation of cells in vitro

4. growing plants on smaller scale

Subtopic:  Plant Tissue Culture |
 69%
From NCERT
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Protoplast is

1. another name for protoplasm

2. an animal cell

3. a plant cell without a cell wall

4. a plant cell

Subtopic:  Plant Tissue Culture |
 83%
From NCERT
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To isolate protoplast, one needs

1. pectinase

2. cellulase

3. both pectinase and cellulase

4. chitinase

Subtopic:  Plant Tissue Culture |
 84%
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