Assertion (A): | It is desirable to use restriction enzymes that make asymmetrical cuts in construction of rDNA molecules. |
Reason (R): | Formation of asymmetrical cuts facilitates the function of DNA ligase. |
1. | Both (A) and (R) are True and (R) correctly explains (A). |
2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
3. | Both (A) and (R) are False. |
4. | (A) is True, (R) is False. |
I: | They cleave dsDNA into fragments at specific recognition sites within molecules known as restriction sites. |
II: | These enzymes are found in bacteria and provide a defense mechanism against invading viruses. |
Assertion (A): | Restriction enzymes are found in bacteria and provide a defense mechanism against invading viruses. |
Reason (R): | They interact with membranes of the host bacterial cells and remove all the receptor sites by the viruses. |
1. | Both (A) and (R) are True and (R) correctly explains (A). |
2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
3. | (A) is True; (R) is False |
4. | Both (A) and (R) are False |
1. | Cleaving of phosphodiester bond in DNA by the enzyme |
2. | Cutting of DNA at specific position only |
3. | Prevention of the multiplication of bacteriophage by the host bacteria |
4. | All of the above |
Assertion (A): | Foreign and vector DNA must be cleaved with the same restriction enzyme when making recombinant DNA. |
Reason (R): | Each restriction enzyme cuts DNA at a specific cleavage site. |
1. | Both (A) and (R) are True and (R) correctly explains (A). |
2. | (A) is True; (R) is False |
3. | (A) is False; (R) is True |
4. | Both (A) and (R) are True but (R) does not correctly explain (A). |