- 1(current)
Q. No.Select the correct match w.r.t steps involved in PCR.
| 1. | Denaturation | - | Phosphodiester bonds break |
| 2. | Annealing | - | Primers anneal at 5' end of DNA template |
| 3. | Extension | - | Taq polymerase forms hydrogen bonds between adjacent nucleotides of growing DNA strand |
| 4. | Extension | - | Thermostable DNA polymerase adds nucleotide to 3' end of primers |
Subtopic: Â Polymerase Chain Reaction: PCR |
From NCERT
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| Assertion (A): | Synthetic oligonucleotide polymers are used during annealing in a PCR. |
| Reason (R): | The primers bind to the double stranded DNA at their complementary regions. |
| 1. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are True and (R) is not the correct explanation of (A). |
| 3. | (A) is True but (R) is False. |
| 4. | (A) is False but (R) is True. |
Subtopic: Â Polymerase Chain Reaction: PCR |
From NCERT
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Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R).
| Assertion (A): | The use of Taq polymerase has greatly facilitated the amplification of DNA by Polymerase Chain Reaction. |
| Reason (R): | Taq polymerase is a DNA polymerase. |
| 1. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
| 3. | (A) is True but (R) is False. |
| 4. | (A) is False but (R) is True. |
Subtopic: Â Polymerase Chain Reaction: PCR |
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From NCERT
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Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
1. Denaturation of template DNA
2. Annealing of primers to template DNA
3. Extension of primer end on the template DNA
4. All of the above
Subtopic: Â Polymerase Chain Reaction: PCR |
 56%
From NCERT
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NEET 2026 - Target Batch - Vital
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NEET 2026 - Target Batch - Vital
- 1(current)
Q. No.
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