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Q. No.Identify the incorrect statement regarding restriction endonucleases used in rDNA technology?
I:
They cleave dsDNA into fragments at specific recognition sites within molecules known as restriction sites.
II:
These enzymes are found in bacteria and provide a defense mechanism against invading viruses.
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Â Restriction Enzymes - Main Enzymes |
From NCERT
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What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism?
Choose the correct answer from the options given below:
| A: | The piece of DNA would be able to multiply itself independently in the progeny cells of the organism. |
| B: | It may get integrated into the genome of the recipient. |
| C: | It may multiply and be inherited along with the host DNA. |
| D: | The alien piece of DNA is not an integral part of chromosome. |
| E: | It shows ability to replicate. |
| 1. | D and E only | 2. | B and C only |
| 3. | A and E only | 4. | A and B only |
Subtopic: Â Principles of Biotechnology |
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Which of the following is required in a cloning vector?
| 1. | Origin of replication, a selectable marker, and a resistance gene |
| 2. | Origin of replication, a selectable marker, and a foreign DNA insert |
| 3. | A gene for replication, a selectable marker, and an antibiotic resistance gene |
| 4. | Origin of replication, a high copy number, and an antibiotic resistance gene |
Subtopic: Â Cloning Vector |
From NCERT
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Consider the given two statements:
| Assertion (A): | It is desirable to use restriction enzymes that make asymmetrical cuts in construction of rDNA molecules. |
| Reason (R): | Formation of asymmetrical cuts facilitates the function of DNA ligase. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | Both (A) and (R) are False. |
| 4. | (A) is True, (R) is False. |
Subtopic: Â Restriction Enzymes - Main Enzymes |
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From NCERT
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Consider the given two statements:
| Assertion (A): | Plasmid DNA is used to monitor bacterial transformation with foreign DNA. |
| Reason (R): | Plasmid is extrachromosomal DNA in some bacteria. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A) |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A) |
| 3. | (A) is True but (R) is False |
| 4. | (A) is False but (R) is True |
Subtopic: Â Cloning Vector |
From NCERT
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'Restriction' in Restriction enzyme refers to:
| 1. | Cleaving of phosphodiester bond in DNA by the enzyme |
| 2. | Cutting of DNA at specific position only |
| 3. | Prevention of the multiplication of bacteriophage by the host bacteria |
| 4. | All of the above |
Subtopic: Â Restriction Enzymes - Main Enzymes |
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The process by which a virus transfers genetic material from one bacterium to another is called:
| 1. | Transduction | 2. | Conjugation |
| 3. | Transformation | 4. | Translation |
Subtopic: Â Tools |
From NCERT
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Consider the given two statements:
| Assertion(A): | Any piece of DNA when linked to Ori sequence of a suitable vector can be made to replicate within the host cells. |
| Reason (R): | Ori sequence is responsible for controlling the copy number of the linked DNA. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True; (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Â Cloning Vector |
From NCERT
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Consider the given two statements:
| Assertion (A): | Restriction enzymes are found in bacteria and provide a defense mechanism against invading viruses. |
| Reason (R): | They interact with membranes of the host bacterial cells and remove all the receptor sites by the viruses. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True; (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Â Restriction Enzymes - Main Enzymes |
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From NCERT
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Consider the two given statements:
| Assertion (A): | Foreign and vector DNA must be cleaved with the same restriction enzyme when making recombinant DNA. |
| Reason (R): | Each restriction enzyme cuts DNA at a specific cleavage site. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | (A) is True; (R) is False |
| 3. | (A) is False; (R) is True |
| 4. | Both (A) and (R) are True but (R) does not correctly explain (A). |
Subtopic: Â Restriction Enzymes - Main Enzymes |
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