Consider the given two statements:
Assertion (A): Restriction enzymes are found in bacteria and provide a defense mechanism against invading viruses.
Reason (R): They interact with membranes of the host bacterial cells and remove all the receptor sites by the viruses.
 
1. Both (A) and (R) are True and (R) correctly explains (A).
2. Both (A) and (R) are True but (R) does not correctly explain (A).
3. (A) is True; (R) is False
4. Both (A) and (R) are False

Subtopic:  Restriction Enzymes - Main Enzymes |
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From NCERT
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Consider the given two statements:
Assertion(A): Any piece of DNA when linked to Ori sequence of a suitable
vector can be made to replicate within the host cells.
Reason (R): Ori sequence is responsible for controlling the copy number of the linked DNA.
 
1. Both (A) and (R) are True and (R) correctly explains (A).
2. Both (A) and (R) are True but (R) does not correctly explain (A).
3. (A) is True; (R) is False
4. Both (A) and (R) are False
Subtopic:  Cloning Vector |
From NCERT
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The process by which a virus transfers genetic material from one bacterium to another is called:
1. Transduction 2. Conjugation
3. Transformation 4. Translation
Subtopic:  Tools |
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'Restriction' in Restriction enzyme refers to:
1. Cleaving of phosphodiester bond in DNA by the enzyme
2. Cutting of DNA at specific position only
3. Prevention of the multiplication of bacteriophage by the host bacteria
4. All of the above
Subtopic:  Restriction Enzymes - Main Enzymes |
From NCERT
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Consider the two given statements:
Assertion (A):  Foreign and vector DNA must be cleaved with the same restriction enzyme when making recombinant DNA.
Reason (R): Each restriction enzyme cuts DNA at a specific cleavage site.
 
1. Both (A) and (R) are True and (R) correctly explains (A).
2. (A) is True; (R) is False
3. (A) is False; (R) is True
4. Both (A) and (R) are True but (R) does not correctly explain (A).
Subtopic:  Restriction Enzymes - Main Enzymes |
From NCERT
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A plasmid has two genes providing resistance to antibiotics A and B respectively. A foreign DNA has been inserted within the gene for resistance to antibiotic A:
I. Non-Transformants will not survive in a medium containing antibiotic A and in a medium containing antibiotic B.
II. Recombinant transformants will survive in a medium containing antibiotic B but not in a medium containing antibiotic A.
III. Non-recombinant transformants will survive in a medium containing antibiotic A and in a medium containing antibiotic B.
1. Only I and II are correct
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
Subtopic:  Tools: Vectors: I |
From NCERT
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Which of the following can replicate within bacterial cells independent of the control of chromosomal DNA?
I: Plasmid
II: Phage DNA
 
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic:  Cloning Vector |
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From NCERT
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Consider the given two statements:
Assertion (A): Plasmid DNA is used to monitor bacterial transformation with foreign DNA.
Reason (R): Plasmid is extrachromosomal DNA in some bacteria.
 
1. Both (A) and (R) are True and (R) correctly explains (A)
2. Both (A) and (R) are True but (R) does not correctly explain (A)
3. (A) is True but (R) is False 
4. (A) is False but (R) is True
Subtopic:  Cloning Vector |
From NCERT
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Consider the given two statements:
Assertion (A): It is desirable to use restriction enzymes that make asymmetrical cuts in construction of rDNA molecules.
Reason (R): Formation of asymmetrical cuts facilitates the function of DNA ligase.
 
1. Both (A) and (R) are True and (R) correctly explains (A).
2. Both (A) and (R) are True but (R) does not correctly explain (A).
3. Both (A) and (R) are False.
4. (A) is True, (R) is False.
Subtopic:  Restriction Enzymes - Main Enzymes |
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From NCERT
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Assertion (A): Synthetic oligonucleotide polymers are used during annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
 
1. Both (A) and (R) are True and (R) is the correct explanation of (A).
2. Both (A) and (R) are True and (R) is not the correct explanation of (A).
3. (A) is True but (R) is False.
4. (A) is False but (R) is True.
Subtopic:  Polymerase Chain Reaction: PCR |
From NCERT
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