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Q. No.Consider the given two statements:
Assertion: (A)
We cannot see pure DNA fragments in the visible light under microscope.
Reason: (R)
The DNA fragments separate according to their shape only and are too small to be seen in the gel electrophoresis.
1.
Both (A) and (R) are True and the (R) is the correct explanation of the (A).
2.
Both (A) and (R) are True and the (R) is not the correct explanation of the (A).
3.
(A) is True but (R) is False.
4.
Both (A) and (R) are False.
Subtopic: Separation and Isolation of DNA fragments |
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Consider the given two statements:
| Assertion (A): | One antibiotic resistance gene of vector pBR322 helps in selecting the transformants and the other one in selecting recombinants. |
| Reason (R): | The non-recombinants can grow on both antibiotic-rich media while the recombinants grow only on single medium due to insertional inactivation. |
| 1. | Both (A) and (R) are True and the (R) is the correct explanation of the (A). |
| 2. | Both (A) and (R) are True and the (R) is not the correct explanation of the (A). |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are False. |
Subtopic: Cloning Vector |
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Select the correct match w.r.t steps involved in PCR.
| 1. | Denaturation | - | Phosphodiester bonds break |
| 2. | Annealing | - | Primers anneal at 5' end of DNA template |
| 3. | Extension | - | Taq polymerase forms hydrogen bonds between adjacent nucleotides of growing DNA strand |
| 4. | Extension | - | Thermostable DNA polymerase adds nucleotide to 3' end of primers |
Subtopic: Polymerase Chain Reaction: PCR |
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| Assertion (A): | Synthetic oligonucleotide polymers are used during annealing in a PCR. |
| Reason (R): | The primers bind to the double stranded DNA at their complementary regions. |
| 1. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are True and (R) is not the correct explanation of (A). |
| 3. | (A) is True but (R) is False. |
| 4. | (A) is False but (R) is True. |
Subtopic: Polymerase Chain Reaction: PCR |
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Match List-I with List-II
Choose the correct answer from the options given below:
| List-I | List-II | ||
| (A) | Kanamycin | (I) | Delivers genes into animal cells |
| (B) | ClaI | (II) | Selectable marker |
| (C) | Disarmed retroviruses | (III) | Restriction site |
| (D) | Kanamycin Rgene | (IV) | Antibiotic resistance |
Choose the correct answer from the options given below:
| Options: | (A) | (B) | (C) | (D) |
| 1. | (II) | (III) | (I) | (IV) |
| 2. | (III) | (I) | (IV) | (II) |
| 3. | (IV) | (III) | (I) | (II) |
| 4. | (II) | (IV) | (I) | (III) |
Subtopic: Cloning Vector |
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From NCERT
NEET - 2023
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NEET 2026 - Target Batch - Vital
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NEET 2026 - Target Batch - Vital
Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
Choose the correct answer from the options given below:
| A. | Insertion of recombinant DNA into the host cell. |
| B. | Cutting of DNA at specific location by restriction enzyme. |
| C. | Isolation of desired DNA fragment. |
| D. | Amplification of gene of interest using PCR. |
| 1. | B, D, A, C | 2. | B, C, D, A |
| 3. | C, A, B, D | 4. | C, B, D, A |
Subtopic: Process of Biotech |
From NCERT
NEET - 2023
To view explanation, please take trial in the course.
NEET 2026 - Target Batch - Vital
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NEET 2026 - Target Batch - Vital
Which of the following can replicate within bacterial cells independent of the control of chromosomal DNA?
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
| I: | Plasmid |
| II: | Phage DNA |
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Cloning Vector |
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From NCERT
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A plasmid has two genes providing resistance to antibiotics A and B respectively. A foreign DNA has been inserted within the gene for resistance to antibiotic A:
1. Only I and II are correct
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
| I. | Non-Transformants will not survive in a medium containing antibiotic A and in a medium containing antibiotic B. |
| II. | Recombinant transformants will survive in a medium containing antibiotic B but not in a medium containing antibiotic A. |
| III. | Non-recombinant transformants will survive in a medium containing antibiotic A and in a medium containing antibiotic B. |
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
Subtopic: Tools: Vectors: I |
From NCERT
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Consider the two given statements:
| Assertion (A): | Foreign and vector DNA must be cleaved with the same restriction enzyme when making recombinant DNA. |
| Reason (R): | Each restriction enzyme cuts DNA at a specific cleavage site. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | (A) is True; (R) is False |
| 3. | (A) is False; (R) is True |
| 4. | Both (A) and (R) are True but (R) does not correctly explain (A). |
Subtopic: Restriction Enzymes - Main Enzymes |
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| Assertion (A): | In order to force bacteria to take up the plasmid, the bacterial cells must be ‘competent’ to take up DNA. |
| Reason (R): | No bacteria have natural competence. |
| 1. | (A) is True but (R) is False. |
| 2. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 3. | (A) is False and (R) is True. |
| 4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
Subtopic: Transforming Plant & Animal Cell |
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