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Q. No.Consider the following two statements:
I:
Plasmid can be used as a vector in recombinant DNA technology experiments.
II:
Plasmid is an extrachromosomal, circular, double-stranded DNA found in some bacteria.
1.
Both I and II are correct and II explains I
2.
Both I and II are correct but II does not explain I
3.
I is correct but II is incorrect
4.
Both I and II are incorrect
Subtopic: Tools: Vectors: I | Cloning Vector |
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In a plasmid such as pBR322:
| I: | Ori controls the copy number of DNA per cell |
| II: | Multiple cloning sites are recognition sequences of common restriction enzymes |
| III: | Genes for antibiotic resistance are used as selectable markers |
| 1. | Only I and II are correct |
| 2. | Only I and III are correct |
| 3. | Only II and III are correct |
| 4. | I, II, and III are correct |
Subtopic: Cloning Vector |
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Given below are two statements: One is labelled as Assertion (A) and the other is labelled as Reason (R).
| Assertion (A): | In rDNA technology, non-recombinants transformed bacteria grow on the medium containing ampicillin as well as the medium containing tetracycline. |
| Reason (R): | Recombinant plasmids contain the foreign gene of interest. |
In the light of the above statements, choose the correct answer from the options given below:
| 1. | (A) is True but (R) is False. |
| 2. | (A) is False but (R) is True. |
| 3. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
Subtopic: General Design of an rDNA experiment |
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| Assertion (A): | In order to force bacteria to take up the plasmid, the bacterial cells must be ‘competent’ to take up DNA. |
| Reason (R): | No bacteria have natural competence. |
| 1. | (A) is True but (R) is False. |
| 2. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 3. | (A) is False and (R) is True. |
| 4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
Subtopic: Transforming Plant & Animal Cell |
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Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
Choose the correct answer from the options given below:
| A. | Insertion of recombinant DNA into the host cell. |
| B. | Cutting of DNA at specific location by restriction enzyme. |
| C. | Isolation of desired DNA fragment. |
| D. | Amplification of gene of interest using PCR. |
| 1. | B, D, A, C | 2. | B, C, D, A |
| 3. | C, A, B, D | 4. | C, B, D, A |
Subtopic: Process of Biotech |
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NEET - 2023
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Match List-I with List-II
Choose the correct answer from the options given below:
| List-I | List-II | ||
| (A) | Kanamycin | (I) | Delivers genes into animal cells |
| (B) | ClaI | (II) | Selectable marker |
| (C) | Disarmed retroviruses | (III) | Restriction site |
| (D) | Kanamycin Rgene | (IV) | Antibiotic resistance |
Choose the correct answer from the options given below:
| Options: | (A) | (B) | (C) | (D) |
| 1. | (II) | (III) | (I) | (IV) |
| 2. | (III) | (I) | (IV) | (II) |
| 3. | (IV) | (III) | (I) | (II) |
| 4. | (II) | (IV) | (I) | (III) |
Subtopic: Cloning Vector |
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Consider the given two statements:
| Assertion: (A) | We cannot see pure DNA fragments in the visible light under microscope. |
| Reason: (R) | The DNA fragments separate according to their shape only and are too small to be seen in the gel electrophoresis. |
| 1. | Both (A) and (R) are True and the (R) is the correct explanation of the (A). |
| 2. | Both (A) and (R) are True and the (R) is not the correct explanation of the (A). |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are False. |
Subtopic: Separation and Isolation of DNA fragments |
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Consider the given two statements:
| Assertion (A): | One antibiotic resistance gene of vector pBR322 helps in selecting the transformants and the other one in selecting recombinants. |
| Reason (R): | The non-recombinants can grow on both antibiotic-rich media while the recombinants grow only on single medium due to insertional inactivation. |
| 1. | Both (A) and (R) are True and the (R) is the correct explanation of the (A). |
| 2. | Both (A) and (R) are True and the (R) is not the correct explanation of the (A). |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are False. |
Subtopic: Cloning Vector |
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Select the correct match w.r.t steps involved in PCR.
| 1. | Denaturation | - | Phosphodiester bonds break |
| 2. | Annealing | - | Primers anneal at 5' end of DNA template |
| 3. | Extension | - | Taq polymerase forms hydrogen bonds between adjacent nucleotides of growing DNA strand |
| 4. | Extension | - | Thermostable DNA polymerase adds nucleotide to 3' end of primers |
Subtopic: Polymerase Chain Reaction: PCR |
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| Assertion (A): | Synthetic oligonucleotide polymers are used during annealing in a PCR. |
| Reason (R): | The primers bind to the double stranded DNA at their complementary regions. |
| 1. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are True and (R) is not the correct explanation of (A). |
| 3. | (A) is True but (R) is False. |
| 4. | (A) is False but (R) is True. |
Subtopic: Polymerase Chain Reaction: PCR |
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