DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

1.	centrifugation
2.	polymerase chain reaction
3.	electrophoresis
4.	restriction mapping

Which of the following is not correctly matched for the organism and its cell wall

degrading enzyme?

1. Bacteria —Lysozyme

2. Plant cells — Cellulase

3. Algae —Methylase

4. Fungi — Chitinase

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of 

Which one is a true statement regarding DNA polymerase used in PCR?

1. It is used to ligate introduced DNA in recipient cells

2. It serves as a selectable marker

3. It is isolated from a virus

4. It remains active at high temperature

For transformation, microparticles coated with DNA to be bombarded with gene gun are

made up of

1. silver or platinum

2. platinum or zinc

3. silicon or platinum

4. gold or tungsten

Given below is a sample of portion of DNA strand giving the base sequence on the

opposite strands. What is so, special shown in it?

5'__ GAATTC_ 3'

3' __ CTTAAG__5'

1. Deletion mutation

2. Start codon at the 5' end

3. Palindromic sequence of base pairs

4. Replication completed

There is a restriction endonuclease called Eco RI. What does 'co' part in it stand for?

1. Coelom                         

2. Coenzyme

3. coli                               

4. Colon

Agarose extracted from sea weeds finds use in

1. tissue culture

2. PCR

3. gel electrophoresis

4. spectrophotometry

Which one of the following is used as vector for cloning genes into higher organisms?

1. Baculovirus

2. Salmonella typhimurium

3. Rhizopus nigricans

4. Retrovirus

Which one of the following palindromic base sequences in DNA can be easily cut at about

the middle by some particular restriction enzyme?

1. 5'$-$CGTTCG$-$3'

     3'$-$ATGGTA$-$-5'

2. 5'$-$GATATG$-$3'

     3'$-$CTACTA$-$5'

3. 5'$-$GAATTC$-$3'

     3'$-$CTTAAG$-$5'

4. 5'$-$CACGTA$-$3'

     3'$-$CTCAGT$-$5'