| 1. | Has a higher risk of causing allergies compared to pig insulin. |
| 2. | Is exactly the same as insulin produced in the human pancreas. |
| 3. | Is produced by inserting the human insulin gene into bacteria. |
| 4. | Requires human cells for production. |
| (a) | Pro-hormone insulin contain extra stretch of C-peptide |
| (b) | A-peptide and B-peptide chains of insulin were produced separately in E.coli, extracted and combined by creating disulphide bond between them. |
| (c) | Insulin used for treating Diabetes was extracted from Cattles and Pigs. |
| (d) | Pro-hormone Insulin needs to be processed for converting into a mature and functional hormone. |
| (e) | Some patients develop allergic reactions to the foreign insulin. |
| 1. | (c), (d) and (e) only | 2. | (a), (b) and (d) only |
| 3. | (b) only | 4. | (c) and (d) only |
| Assertion(A): | The main challenge for production of insulin using rDNA techniques was getting insulin assembled into a mature form. |
| Reason (R): | Insulin consists of two short polypeptide chains: chain A and chain B, that are linked together by disulphide bridges. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True; (R) is False |
| 4. | Both (A) and (R) are False |
| Assertion(A): | Insulin isolated from other cattle or pigs is not as effective as that secreted by the human body itself. |
| Reason (R): | Insulin isolated from other cattle or pigs will elicit an immune response in the human body |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True; (R) is False |
| 4. | Both (A) and (R) are False |
| I: | All diabetics require insulin from the very beginning. |
| II: | Insulin can be given orally to diabetics. |
| III. | Insulin extracted from pancreas of slaughtered cattle and pigs caused some patients to develop allergy. |
| IV. | Recombinant insulin was first prepared by Indian pharmaceutical giant Ranbaxy. |
| V. | The two polypeptide chains in the final structure of Insulin are joined by disulfide bonds. |
| VI. | DNA sequences corresponding to A and B chains of human insulin were introduced in plasmids of E.coli to produce insulin chains for preparing recombinant insulin. |
| 1. | 2 | 2. | 3 |
| 3. | 4 | 4. | 5 |