Q. No.| 1. | Janus green followed by exposure to gamma radiation |
| 2. | Acetocarmine followed by exposure to infrared radiation |
| 3. | Ethidium bromide followed by exposure to UV radiation |
| 4. | Methylene blue followed by exposure to X-rays |
| 1. | DNA is positively charged molecules |
| 2. | DNA moves in random direction |
| 3. | Anode is negatively charged |
| 4. | DNA is negatively charged molecules |
In-gel electrophoresis, the differential mobility of DNA depends on_______
| 1. | Helical nature of DNA |
| 2. | Double-stranded nature of DNA |
| 3. | Charge and size of DNA |
| 4. | Hydrogen bonding between bases |
Agarose extracted from sea weeds finds use in:
1. Spectrophotometry
2. Tissue Culture
3. PCR
4. Gel electrophoresis
DNA strands on a gel stained with ethidium bromide, when viewed under UV radiation, appear as:
1. Dark red bands
2. Bright blue bands
3. Yellow bands
4. Bright orange bands
Mark the incorrect statement
1. Bam HI restriction site is present in tetracycline resistant part of pBR-322
2. Separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide
3. Agrobacterium tumefaciens is considered as natural genetic engineer
4. In the gel electrophoresis DNA fragments resolve according to base sequence
A. Isolation of foreign DNA
B. Restriction digestion of foreign DNA and plasmid vector
C. Ligation of DNA to plasmid
D. Blue white selection
E. Transformation in cloning host
Choose the correct answer from the options given below:
1. A>B>C>E>D
2. A>B>E>C>D
3. A>C>B>E>D
4. A>D>E>B>C
DNA precipitation out of a mixture of biomolecules can be achieved by treatment with:
| 1. | Chilled chloroform |
| 2. | isopropanal |
| 3. | Chilled ethanol |
| 4. | Methanol at room temperature |
Which of the following is not correct for the organism and its cell wall degrading enzyme?
| 1. | bacteria - lysozyme |
| 2. | plant cells - cellulase |
| 3. | algae - methylase |
| 4. | fungi - chitinase |
| Assertion: | During isolation of genetic material (DNA) chilled ethanol is used. |
| Reason: | Chilled ethanol precipitates out DNA which can be observed as collection of fine threads in the suspension. |
| 1. | Both Assertion and Reason are true and the Reason is the correct explanation of the Assertion. |
| 2. | Both Assertion and Reason are true and the Reason is not the correct explanation of the Assertion. |
| 3. | Assertion is true but Reason is false. |
| 4. | Both Assertion and Reason are false. |
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