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Q. No.The two core techniques that enabled the birth of modern biotechnology are:
1.
hybridisation and mutation breeding
2.
genetic engineering and bioprocess engineering
3.
tissue culture and cloning
4.
fermentation and enzyme technology
Subtopic: Principles of Biotechnology |
94%
Level 1: 80%+
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Genetic engineering offers an advantage over traditional breeding primarily because it:
| 1. | increases mutation rate in organisms |
| 2. | eliminates requirement of host organisms |
| 3. | bypasses need for vectors |
| 4. | allows transfer of only selected desirable genes |
Subtopic: Principles of Biotechnology |
89%
Level 1: 80%+
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DNA cannot easily pass through the bacterial cell membrane mainly because DNA is:
| 1. | very small | 2. | hydrophilic |
| 3. | positively charged | 4. | lipid-soluble |
Subtopic: Transforming Plant & Animal Cell |
83%
Level 1: 80%+
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A scientist starts a PCR (Polymerase Chain Reaction) experiment with a single copy of a target DNA molecule. After completing 20 cycles of PCR, how many copies of the target DNA will be present?
| 1. | 20 copies | 2. | 400 copies |
| 3. | 20² copies | 4. | 2²⁰ copies |
Subtopic: Polymerase Chain Reaction: PCR |
80%
Level 1: 80%+
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The bacterium Agrobacterium tumefaciens is often used in plant genetic engineering because it:
| 1. | Contains Ti plasmid that can transfer foreign genes into plants. |
| 2. | Naturally produces insecticidal proteins. |
| 3. | Has the ability to fix atmospheric nitrogen. |
| 4. | Can degrade organic pollutants in the soil. |
Subtopic: Cloning Vector |
95%
Level 1: 80%+
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The main function of the flat-bladed impeller in the bioreactor shown in the figure is to:
1. Control the pH of the culture medium
2. Mix the contents uniformly and maintain oxygen transfer
3. Supply sterile air to the culture broth
4. Break the foam formed during fermentation
1. Control the pH of the culture medium
2. Mix the contents uniformly and maintain oxygen transfer
3. Supply sterile air to the culture broth
4. Break the foam formed during fermentation
Subtopic: Bioreactors/Downstream Processing |
84%
Level 1: 80%+
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What makes continuous culture systems more advantageous than batch culture?
1. Lower contamination risk
2. Steady state growth conditions
3. Cheaper operation costs
4. Simpler equipment requirements
1. Lower contamination risk
2. Steady state growth conditions
3. Cheaper operation costs
4. Simpler equipment requirements
Subtopic: Bioreactors/Downstream Processing |
75%
Level 2: 60%+
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In a bioreactor, the stirrer serves to:
| 1. | Only maintain temperature |
| 2. | Only provide aeration |
| 3. | Both maintain temperature and provide aeration |
| 4. | Neither maintain temperature nor provide aeration |
Subtopic: Bioreactors/Downstream Processing |
85%
Level 1: 80%+
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The most important parameter monitored in a bioreactor is:
1. Light intensity
2. Dissolved oxygen
3. Carbon dioxide level
4. Pressure only
1. Light intensity
2. Dissolved oxygen
3. Carbon dioxide level
4. Pressure only
Subtopic: Bioreactors/Downstream Processing |
87%
Level 1: 80%+
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The main advantage of continuous culture system in bioreactors is:
| 1. | Lower maintenance cost |
| 2. | Constant supply of fresh medium and removal of waste |
| 3. | Higher temperature control |
| 4. | Better aeration only |
Subtopic: Bioreactors/Downstream Processing |
91%
Level 1: 80%+
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