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Q. No.The separated DNA fragments can be visualised only after staining the DNA with a compound known as:
1.
Janus green followed by exposure to gamma radiation
2.
Acetocarmine followed by exposure to infrared radiation
3.
Ethidium bromide followed by exposure to UV radiation
4.
Methylene blue followed by exposure to X-rays
Subtopic: Separation and Isolation of DNA fragments |
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Which of the following are the features that are required to facilitate cloning into a vector?
A: Origin of replication (ori)
B: Selectable marker
C: Cloning sites
1. Only A and B
2. Only A and C
3. Only B and C
4. A, B and C
A: Origin of replication (ori)
B: Selectable marker
C: Cloning sites
1. Only A and B
2. Only A and C
3. Only B and C
4. A, B and C
Subtopic: Cloning Vector |
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In the given figure of the vector pBR322, which alphabet represents the location of the gene for resistance to tetracycline?
1. A
2. B
3. C
4. D
1. A
2. B
3. C
4. D
Subtopic: Cloning Vector |
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After cutting both the source DNA containing the gene of interest and the vector DNA with the same restriction enzyme, they are mixed and DNA ligase is added. The use of the same restriction enzyme is crucial because it:
| 1. | Increases the length of the gene of interest |
| 2. | Produces identical and complementary ends on both DNAs, allowing them to join |
| 3. | Ensures the ligase functions only on source DNA |
| 4. | Prevents the gene of interest from undergoing transcription |
Subtopic: Tools: Enzymes: I | Tools: Enzymes: II |
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Besides better aeration and mixing properties, what is another advantage of stirred-tank bioreactors over shake flasks?
| 1. | They provide better control over pH, temperature, and nutrient supply during fermentation. |
| 2. | They are simpler and more cost-effective than shake flasks. |
| 3. | They are only suitable for small-scale culture experiments. |
| 4. | They do not require continuous monitoring or adjustments. |
Subtopic: Bioreactors/Downstream Processing |
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Why are restriction enzymes referred to as "molecular scissors"?
| 1. | Because they randomly degrade all DNA molecules inside a cell. |
| 2. | Because they attach new DNA segments to a target sequence during genetic recombination. |
| 3. | Because they cut DNA at specific recognition sequences, allowing precise genetic modifications. |
| 4. | Because they unwind and separate DNA strands during replication. |
Subtopic: Restriction Enzymes - Main Enzymes |
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In genetic engineering, an alien piece of DNA must be linked to a specific sequence in a chromosome called the origin of replication to ensure its multiplication in the host organism. What is the primary function of the origin of replication?
| 1. | It provides a binding site for ribosomes to initiate translation. |
| 2. | It serves as the site where DNA replication begins, allowing the foreign DNA to replicate along with the host genome. |
| 3. | It facilitates the transcription of the alien DNA into mRNA for protein synthesis. |
| 4. | It acts as a recognition site for restriction enzymes to cut and insert new DNA segments. |
Subtopic: Cloning Vector |
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In the restriction enzyme EcoRI, the letter 'R' is derived from :
| 1. | Genus name of the source organism |
| 2. | Species name of the source organism |
| 3. | Strain designation of the source organism |
| 4. | Order of discovery in the species |
Subtopic: Restriction Enzymes - Main Enzymes |
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Techniques that alter the chemistry of genetic material (DNA and RNA), introduce them into host organisms, and consequently modify the phenotype of the host are part of which field of study?
1. Bioprocess Engineering
2. Tissue Culture
3. Genetic Engineering
4. Molecular Taxonomy
1. Bioprocess Engineering
2. Tissue Culture
3. Genetic Engineering
4. Molecular Taxonomy
Subtopic: Principles of Biotechnology |
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Bacteria naturally produce restriction endonucleases as a mechanism to:
| 1. | Defend against viral invasion by cleaving non-methylated DNA sequences |
| 2. | Degrade foreign RNA molecules within their cytoplasm |
| 3. | Facilitate horizontal gene transfer through transformation |
| 4. | Repair mismatched DNA during replication |
Subtopic: Restriction Enzymes - Main Enzymes | Tools |
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