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Q. No.During agarose gel electrophoresis DNA fragments moves towards anode. Because-
1.
DNA is positively charged molecules
2.
DNA moves in random direction
3.
Anode is negatively charged
4.
DNA is negatively charged molecules
Subtopic: Separation and Isolation of DNA fragments |
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The most important feature in a plasmid to be used as a vector is-
1. Origin of replication (ori)
2. Presence of a selectable marker
3. Presence of sites for restriction endonuclease
4. Its size
1. Origin of replication (ori)
2. Presence of a selectable marker
3. Presence of sites for restriction endonuclease
4. Its size
Subtopic: Cloning Vector |
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Which of the following has popularized the Polymerase Chain Reaction?
| 1. | Easy availability of DNA template |
| 2. | Availability of synthetic primers |
| 3. | Availability of cheap deoxy ribonucleotides |
| 4. | Availability of ' Thermostable ' DNA polymerase |
Subtopic: Polymerase Chain Reaction: PCR |
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For transformation, micro particles coated with DNA to be bombarded with gene gun are made up of-
1. Silver or Platinum
2. Gold or Tungsten
3. Silicon and Platinum
4. Platinum or Zinc
1. Silver or Platinum
2. Gold or Tungsten
3. Silicon and Platinum
4. Platinum or Zinc
Subtopic: Transforming Plant & Animal Cell |
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| Assertion(A): | Insertion of recombinant DNA within the coding sequence of β-galactosidase results in colourless colonies. |
| Reason(R): | Presence of insert results in inactivation of enzyme β-galactosidase known as insertional inactivation. |
| 1. | Both (A) and (R) are True and (R) is the correct explanation of (A) |
| 2. | Both (A) and (R) are True and (R) is not the correct explanation of (A) |
| 3. | (A) is True but (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Cloning Vector |
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Which of the following correctly describes the mechanism by which DNA enters bacterial cells after being treated with divalent cations and subjected to heat shock?
| 1. | The divalent cations directly bind to DNA, forcing it into the bacterial cells under heat shock. |
| 2. | Heat shock enhances the replication of plasmid DNA, allowing it to enter the cells without the need for cations. |
| 3. | Divalent cations reduce the hydrophilicity of DNA, while heat shock stabilizes the bacterial cell wall. |
| 4. | The divalent cations increase the permeability of the cell membrane, while heat shock creates transient pores for DNA entry. |
Subtopic: Transforming Plant & Animal Cell |
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Consider the given two statements:
Options:
| (a) | Asexual reproduction preserves the genetic information, while sexual reproduction introduces variations. |
| (b) | Genetic engineering allows the transfer of only desirable genes, avoiding the inclusion of undesirable genes. |
| 1. | Only (a) is correct | 2. | Only (b) is correct |
| 3. | Both (a) and (b) are correct | 4. | Both (a) and (b) are incorrect |
Subtopic: Principles of Biotechnology |
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Why are the sticky ends produced by restriction enzymes important in recombinant DNA technology?
| 1. | They protect the DNA fragments from exonucleases. |
| 2. | They prevent the insertion of DNA fragments into the vector. |
| 3. | They ensure that the DNA fragments cannot be re-ligated by DNA ligase. |
| 4. | They allow fragments with identical sticky ends to hydrogen bond and facilitate ligation. |
Subtopic: Tools |
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Which of the following correctly describes the naming convention for restriction enzymes?
| 1. | The first letter represents the genus, the next two letters represent the species, the strain is indicated by an additional letter, and Roman numerals indicate the order of discovery. |
| 2. | The first letter represents the genus, the next two letters represent the species, and Roman numerals indicate the recognition sequence. |
| 3. | The first three letters represent the bacterial species, followed by the strain name and Roman numerals indicating the recognition sequence. |
| 4. | The entire name of the bacterial strain is used, followed by Roman numerals indicating the enzyme's function. |
Subtopic: Restriction Enzymes - Main Enzymes | Tools |
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Which of the following statements about restriction enzymes is incorrect?
| 1. | Restriction enzymes are also called molecular scissors because they cleave DNA at specific sequences. |
| 2. | They are naturally found in eukaryotic cells to protect against viral DNA. |
| 3. | EcoRI is a restriction enzyme that recognizes the sequence GAATTC. |
| 4. | Restriction enzymes often create sticky ends, facilitating ligation. |
Subtopic: Restriction Enzymes: Historical Background | Tools |
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