The breakthrough work of which of the following scientist duo was the basis upon which the discipline of biotechnology was founded?
1. Nirenberg and Mathai
2. Cohen and Boyer
3. Millstein and Kohler
4. Khurana and Venkatraman

Subtopic:  Principles of Biotechnology |
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Level 1: 80%+
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What was the key contribution of Cohen and Boyer to biotechnology?
1. They discovered the structure of DNA.
2. They developed the first recombinant DNA.
3. They were the first to sequence the human genome.
4. They discovered how to visualize DNA fragments using agarose gel electrophoresis
Subtopic:  Introduction & History: I |
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Level 1: 80%+
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Which statement is true regarding cloning vectors?
1. Plasmids and bacteriophages can both serve as cloning vectors in gene cloning.
2. Cloning vectors are unnecessary in the process of creating recombinant DNA.
3. A vector must be larger than the DNA fragment it is designed to carry.
4. Only animal viruses can be used as vectors in gene cloning processes.
Subtopic:  Cloning Vector |
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Level 1: 80%+
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Consider the given two statements:
Statement I: The most commonly used bioreactors in downstream processing are of stirring type.
Statement II: The stirrer facilitates even mixing and oxygen availability throughout the bioreactor.
1. Only Statement I is correct.
2. Only Statement II is correct.
3. Both Statement I and Statement II are correct.
4. Both Statement I and Statement II are incorrect.
Subtopic:  Bioreactors/Downstream Processing |
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Level 1: 80%+
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Which of the following techniques is commonly used for the production of genetically modified dicot plants? 
1. Polymerase Chain Reaction (PCR) 
2. Southern blotting 
3. Agarose gel electrophoresis 
4. Agrobacterium-mediated gene transfer
Subtopic:  Transforming Plant & Animal Cell |
 85%
Level 1: 80%+
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Ti plasmid is found in:
1. Escherichia coli
2. Agrobacterium tumefaciens
3. Salmonella typhimurium
4. Haemophilus influenzae
Subtopic:  Transforming Plant & Animal Cell |
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Level 1: 80%+
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Restriction enzymes are named:
1. After the scientist who discovered them.
2. After the bacterial species from which they are derived.
3. According to the number of base pairs they cut.
4. Based on the type of DNA they recognize.
Subtopic:  Restriction Enzymes - Main Enzymes |
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Level 1: 80%+
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Why is Taq polymerase used in PCR?
1. It is heat-stable and can withstand the high temperatures of PCR.
2. It is more accurate than other DNA polymerases.
3. It can replicate DNA without a template.
4. It denatures at lower temperatures, speeding up the PCR process.
Subtopic:  Polymerase Chain Reaction: PCR |
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Level 1: 80%+
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Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:
1. 6 bp 2. 4 bp
3. 10 bp 4. 8 bp
Subtopic:  Restriction Enzymes - Main Enzymes | Restriction Enzymes: Historical Background |
 82%
Level 1: 80%+
NEET - 2024
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The " Ti plasmid " of Agrobacterium tumefaciens stands for 
1. Tumor independent plasmid 
2. Tumor inducing plasmid 
3. Temperature independent plasmid 
4. Tumour inhibiting  plasmid
Subtopic:  Tools: Vectors: I |
 83%
Level 1: 80%+
NEET - 2024
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