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Q. No.The extrachromosomal circular double stranded DNA found in some bacterial cells and used in rDNA procedures is:
1.
Genophore
2.
Nucleoid
3.
Plasmid
4.
Pili
Subtopic: Principles of Biotechnology |
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Level 1: 80%+
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Ori
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
| I: | is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells. |
| II: | sequence is also responsible for controlling the copy number of the linked DNA. |
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Cloning Vector |
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Biotechnology is essentially:
| 1. | Manipulation of physical structures |
| 2. | Use of live organisms or enzymes for useful products and processes |
| 3. | Extraction of minerals from organisms |
| 4. | Creation of artificial intelligence |
Subtopic: Introduction & History |
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Level 1: 80%+
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The restriction endonuclease enzymes have been isolated from:
| 1. | Bacteria | 2. | Yeasts |
| 3. | Algae | 4. | Fungi |
Subtopic: Restriction Enzymes - Main Enzymes |
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Consider the given two statements:
1. Only Statement I is correct.
2. Only Statement II is correct.
3. Both Statement I and Statement II are correct.
4. Both Statement I and Statement II are incorrect.
| Statement I: | The most commonly used bioreactors in downstream processing are of stirring type. |
| Statement II: | The stirrer facilitates even mixing and oxygen availability throughout the bioreactor. |
2. Only Statement II is correct.
3. Both Statement I and Statement II are correct.
4. Both Statement I and Statement II are incorrect.
Subtopic: Bioreactors/Downstream Processing |
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The breakthrough work of which of the following scientist duo was the basis upon which the discipline of biotechnology was founded?
1. Nirenberg and Mathai
2. Cohen and Boyer
3. Millstein and Kohler
4. Khurana and Venkatraman
1. Nirenberg and Mathai
2. Cohen and Boyer
3. Millstein and Kohler
4. Khurana and Venkatraman
Subtopic: Principles of Biotechnology |
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Level 1: 80%+
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The common restriction enzyme EcoRI recognizes the palindromic sequence:
1. GAATTC
2. CCCGGG
3. AAGCTT
4. TGGCCA
1. GAATTC
2. CCCGGG
3. AAGCTT
4. TGGCCA
Subtopic: Restriction Enzymes - Main Enzymes |
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Bacterial cells add methyl groups to their DNA in order to:
1. clone their DNA
2. activate promoters
3. form polycisronic mRNAs
4. protect its DNA from own restriction enzymes
1. clone their DNA
2. activate promoters
3. form polycisronic mRNAs
4. protect its DNA from own restriction enzymes
Subtopic: Restriction Enzymes - Main Enzymes |
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In a plasmid cloning vector what is responsible for controlling the copy number of the linked DNA?
1. Ori
2. MCS
3. Selectable marker
4. Promoter
1. Ori
2. MCS
3. Selectable marker
4. Promoter
Subtopic: Tools: Vectors: I |
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In the preparation of recombinant DNA, the cut out ‘gene of interest’ from the source DNA and the cut vector with space are mixed and:
| 1. | ligase is added. |
| 2. | the temperature of the apparatus is increased to 95 degree Celsius. |
| 3. | a suitable host cell is incubated with the fragments. |
| 4. | primers are added for annealing to DNA fragments. |
Subtopic: Process of Biotech |
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