Select Chapter Topics:
Q. No.
Assertion:
During isolation of genetic material (DNA) chilled ethanol is used.
Reason:
Chilled ethanol precipitates out DNA which can be observed as collection of fine threads in the suspension.
1.
Both Assertion and Reason are true and the Reason is the correct explanation of the Assertion.
2.
Both Assertion and Reason are true and the Reason is not the correct explanation of the Assertion.
3.
Assertion is true but Reason is false.
4.
Both Assertion and Reason are false.
Subtopic: Separation and Isolation of DNA fragments |
95%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
The role of sparger in sparged stirred tank bioreactor is to
| 1. | Provide steam for sterilisation |
| 2. | Act as foam braker |
| 3. | Perform the function of flat bladed impeller |
| 4. | Produce bubbles that dramatically increase the O2 transfer area |
Subtopic: Bioreactors/Downstream Processing |
84%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
Select a reporter enzyme that can be used to monitor transformation of host cells by foreign DNA in addition to selectable marker.
1. Protease
2. \(\beta \)-galactosidase
3. Chitinase
4. Lactase
1. Protease
2. \(\beta \)-galactosidase
3. Chitinase
4. Lactase
Subtopic: Selectable Markers |
92%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
In Annealing:
| 1. | Primers anneal to the DNA template |
| 2. | Taq polymerase adds nucleotides to the annealed primer |
| 3. | Two strands of the DNA separate |
| 4. | Temperature is 92ºC |
Subtopic: Polymerase Chain Reaction: PCR |
71%
Level 2: 60%+
Please attempt this question first.
Hints
Please attempt this question first.
Subtopic: Polymerase Chain Reaction: PCR |
94%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
| Assertion (A): | Thermostable polymerase is used in a PCR (Polymerase Chain Reaction). |
| Reason (R): | It remains active at high temperature and causes amplification of DNA during PCR. |
| 1. | Both (A) and (R) are True and (R) is the correct explanation of (A) |
| 2. | Both (A) and (R) are True and (R) is not the correct explanation of (A) |
| 3. | (A) is True, (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Polymerase Chain Reaction: PCR |
95%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
| Assertion(A): | Disarmed plasmid of Agrobacterium tumefaciens is used as a cloning vector. |
| Reason(R): | Disarmed plasmid helps in delivering gene of our interest into monocots only |
| 1. | Both (A) and (R) are true and (R) is the correct explanation of (A) |
| 2. | Both (A) and (R) are true and (R) is not the correct explanation of (A). |
| 3. | (A) is true but (R) is false. |
| 4. | Both (A) and (R) are false. |
Subtopic: Transforming Plant & Animal Cell |
75%
Level 2: 60%+
Please attempt this question first.
Hints
Please attempt this question first.
Isolation of genetic material from fungal cells involves the use of
1. Lysozyme
2. Cellulase
3. Chitinase
4. Polymerase
1. Lysozyme
2. Cellulase
3. Chitinase
4. Polymerase
Subtopic: Introduction & History: I | Introduction & History: II | Process of Biotech | Introduction & History |
90%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
Method in which recombinant DNA is directly injected into the nucleus of animal cell by using microneedles is called
1. Gene Gun method
2. Biolistic method
3. Microinjection method
4. Electroporation
1. Gene Gun method
2. Biolistic method
3. Microinjection method
4. Electroporation
Subtopic: Transforming Plant & Animal Cell | Cloning Vector |
86%
Level 1: 80%+
Please attempt this question first.
Hints
Please attempt this question first.
Which of the following is needed to extract DNA from tomato cells?
1. DNase
2. Lysozyme
3. Cellulase
4. Chitinase
1. DNase
2. Lysozyme
3. Cellulase
4. Chitinase
Subtopic: Host & Desired DNA | Introduction & History | Tools |
77%
Level 2: 60%+
Please attempt this question first.
Hints
Please attempt this question first.
Select Chapter Topics:
© 2026 GoodEd Technologies Pvt. Ltd.