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Q. No.All of the following are a part of biotechnology except:
1.
in vitro fertilisation leading to a ‘test-tube’ baby
2.
synthesising a gene and using it
3.
developing a DNA vaccine or correcting a defective gene
4.
insertion of an IUD for contraception
Subtopic: Introduction & History: I |
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Tetracycline resistance gene in the vector pBR322 carries the recognition sequence for:
| 1. | PstI | 2. | PvuII |
| 3. | BamH I | 4. | EcoR I |
Subtopic: Cloning Vector |
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If an enzyme catalyses the removal of nucleotides from the ends of DNA, then it should be called as:
1. endonuclease
2. exonuclease
3 ligase
4. reverse transcriptase
Subtopic: Restriction Enzymes: Historical Background | Tools |
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The most critical requirement for any DNA molecule to be able to act as a cloning vector during rDNA procedures will be:
1. Ori
2. MCS
3. More than one selectable marker
4. A silent promoter
1. Ori
2. MCS
3. More than one selectable marker
4. A silent promoter
Subtopic: Cloning Vector |
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Thermostable DNA polymerase used in PCR was isolated from:
| 1. | Thermus aquaticus | 2. | Escherichia coli |
| 3. | Agrobacterium tumefaciens | 4. | Bacillus thuringiensis |
Subtopic: Polymerase Chain Reaction: PCR |
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NEET - 2023
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| Assertion(A): | Disarmed plasmid of Agrobacterium tumefaciens is used as a cloning vector. |
| Reason(R): | Disarmed plasmid helps in delivering gene of our interest into monocots only |
| 1. | Both (A) and (R) are true and (R) is the correct explanation of (A) |
| 2. | Both (A) and (R) are true and (R) is not the correct explanation of (A). |
| 3. | (A) is true but (R) is false. |
| 4. | Both (A) and (R) are false. |
Subtopic: Transforming Plant & Animal Cell |
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| Assertion (A): | Thermostable polymerase is used in a PCR (Polymerase Chain Reaction). |
| Reason (R): | It remains active at high temperature and causes amplification of DNA during PCR. |
| 1. | Both (A) and (R) are True and (R) is the correct explanation of (A) |
| 2. | Both (A) and (R) are True and (R) is not the correct explanation of (A) |
| 3. | (A) is True, (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Polymerase Chain Reaction: PCR |
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| Assertion: | During isolation of genetic material (DNA) chilled ethanol is used. |
| Reason: | Chilled ethanol precipitates out DNA which can be observed as collection of fine threads in the suspension. |
| 1. | Both Assertion and Reason are true and the Reason is the correct explanation of the Assertion. |
| 2. | Both Assertion and Reason are true and the Reason is not the correct explanation of the Assertion. |
| 3. | Assertion is true but Reason is false. |
| 4. | Both Assertion and Reason are false. |
Subtopic: Separation and Isolation of DNA fragments |
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The role of sparger in sparged stirred tank bioreactor is to
| 1. | Provide steam for sterilisation |
| 2. | Act as foam braker |
| 3. | Perform the function of flat bladed impeller |
| 4. | Produce bubbles that dramatically increase the O2 transfer area |
Subtopic: Bioreactors/Downstream Processing |
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Select a reporter enzyme that can be used to monitor transformation of host cells by foreign DNA in addition to selectable marker.
1. Protease
2. \(\beta \)-galactosidase
3. Chitinase
4. Lactase
1. Protease
2. \(\beta \)-galactosidase
3. Chitinase
4. Lactase
Subtopic: Selectable Markers |
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