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Q. No.Agarose extracted from sea weeds finds use in:
1. Spectrophotometry
2. Tissue Culture
3. PCR
4. Gel electrophoresis
1. Spectrophotometry
2. Tissue Culture
3. PCR
4. Gel electrophoresis
Subtopic: Separation and Isolation of DNA fragments |
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Given below is a sample of a portion of DNA strand. What is so special shown in it?
5' GAATTC 3'
3' CTTAAG 5'
1. Replication completed
2. Deletion mutation
3. Start codon at the 5' end
4. Palindromic sequence of base pairs
Subtopic: Restriction Enzymes - Main Enzymes |
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The polymerase chain reaction:
| I: | is used to synthesize multiple copies of the gene [or DNA] in vitro. |
| II: | uses a thermostable DNA dependent DNA polymerase. |
| III: | is now routinely used to detect HIV in suspected AIDS patient. |
| IV: | is being used to detect mutations in genes in suspected cancer patients. |
| 1. | All except I are true | 2. | Only II and III are true |
| 3. | Only IV is not true | 4. | I, II, III and IV are true |
Subtopic: Polymerase Chain Reaction: PCR |
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The strain of Escherechia coli from which the restriction enzyme EcoR I has been isolated is:
1. DH5α
2. RY 13
3. NC101
4. K1
1. DH5α
2. RY 13
3. NC101
4. K1
Subtopic: Restriction Enzymes - Main Enzymes |
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The end result of precipitation of DNA in suspension is seen
| 1. | In the form of dark solution |
| 2. | In the form of effervescence |
| 3. | In the form of fine threads |
| 4. | In the form of bubbles |
Subtopic: Host & Desired DNA: II | Host & Desired DNA |
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What does continuous culture mean?
| 1. | Where DNA and protein getting expressed continuously |
| 2. | Where used medium is drained out and the fresh medium is added from another side |
| 3. | Where cells are producing protein for one week continuously |
| 4. | Where the production of recombinant DNA is continuing without interference. |
Subtopic: Large Scale Production: II | Large Scale Production |
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In the diagram showing the plasmid pBR322, the recognition sequence of the restriction enzyme BamHI is shown by the number:
1. 1
2. 2
3. 3
4. 4
Subtopic: Cloning Vector |
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A plasmid has genes for resistance against ampicillin and tetracycline. The tetracycline resistant gene is insertionally inactivated by inserting a foreign DNA within the gene. The bacterial cells are transformed. The recombinant transformants:
| 1. | will be killed in a medium containing ampicillin but not in a medium containing tetracycline |
| 2. | will be killed in a medium containing tetracycline but not in a medium containing ampicillin |
| 3. | will be killed in a medium containing ampicillin and in a medium containing tetracycline |
| 4. | will not be killed in a medium containing ampicillin and in a medium containing tetracycline |
Subtopic: Cloning Vector |
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Gene transfer involves some essential stages such as
The experimental sequence of these events should be
1. (i), (ii), (iii), (iv)
2. (ii), (iv), (i), (iii)
3. (iv), (i), (ii), (iii)
4. (i), (iii), (ii), (iv).
| (i) | inserting recombinant DNA into suitable recipient organism |
| (ii) | isolation of a fragment of DNA |
| (iii) | screening of transformed cells |
| (iv) | integrating DNA fragment into suitable vector |
The experimental sequence of these events should be
1. (i), (ii), (iii), (iv)
2. (ii), (iv), (i), (iii)
3. (iv), (i), (ii), (iii)
4. (i), (iii), (ii), (iv).
Subtopic: Transforming Plant & Animal Cell |
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Consider the following steps which are involved in cloning of foreign DNA into plasmid vector
A. Isolation of foreign DNA
B. Restriction digestion of foreign DNA and plasmid vector
C. Ligation of DNA to plasmid
D. Blue white selection
E. Transformation in cloning host
Choose the correct answer from the options given below:
1. A>B>C>E>D
2. A>B>E>C>D
3. A>C>B>E>D
4. A>D>E>B>C
A. Isolation of foreign DNA
B. Restriction digestion of foreign DNA and plasmid vector
C. Ligation of DNA to plasmid
D. Blue white selection
E. Transformation in cloning host
Choose the correct answer from the options given below:
1. A>B>C>E>D
2. A>B>E>C>D
3. A>C>B>E>D
4. A>D>E>B>C
Subtopic: Restriction Enzymes - Main Enzymes | Separation and Isolation of DNA fragments | Selection of Recombinant Transformants | Obtaining Copy of Gene from Donor DNA |
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