Q. No.Which one of the following techniques made it possible to genetically engineer living organisms?
1. Recombinant DNA techniques
2. X-ray diffraction
3. Heavier isotope labeling
4. Hybridization
1. Recombinant DNA techniques
2. X-ray diffraction
3. Heavier isotope labeling
4. Hybridization
The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain component(s)?
| 1. | ori | original restriction enzyme |
| 2. | rop | reduced osmotic pressure |
| 3. | Hind III, EcoRI | selectable markers |
| 4. | ampR, tetR | antibiotic resistance genes |
| 1. | Ca+2 | 2. | Na+ |
| 3. | K+ | 4. | Cl- |
1. A thermostable DNA polymerase
2. Dideoxy-dNTPs (ddNTPs)
3. Template DNA
4. Primers
The formulation with suitable preservatives in the case of drugs has to undergo:
| 1. | Thorough clinical trials |
| 2. | Thorough strict separation techniques |
| 3. | Thorough harsh heat shock control |
| 4. | Thorough long restriction digestion |
1. Micro-injection
2. Biolistics
3. Gene gun
4. Disarmed pathogen
Which of the following equipments is essentially required for growing microbes on a large scale for industrial production of enzymes?
1. Bioreactor
2. BOD incubator
3. Sludge digester
4. Industrial oven
Agarose extracted from sea weeds finds use in:
1. Spectrophotometry
2. Tissue Culture
3. PCR
4. Gel electrophoresis
Given below is a sample of a portion of DNA strand. What is so special shown in it?
5' GAATTC 3'
3' CTTAAG 5'
1. Replication completed
2. Deletion mutation
3. Start codon at the 5' end
4. Palindromic sequence of base pairs
| I: | is used to synthesize multiple copies of the gene [or DNA] in vitro. |
| II: | uses a thermostable DNA dependent DNA polymerase. |
| III: | is now routinely used to detect HIV in suspected AIDS patient. |
| IV: | is being used to detect mutations in genes in suspected cancer patients. |
| 1. | All except I are true | 2. | Only II and III are true |
| 3. | Only IV is not true | 4. | I, II, III and IV are true |
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