DNA strands on a gel stained with ethidium bromide, when viewed under UV radiation, appear as:
1. Dark red bands
2. Bright blue bands
3. Yellow bands
4. Bright orange bands
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:
| 1. | Palindromic Nucleotide sequences |
| 2. | Poly(A) tail sequences |
| 3. | Degenerate primer sequence |
| 4. | Okazaki sequences |
During the process of gene amplification using PCR, very high temperature is not maintained in the beginning, then which of the following PCR will be affected first?
1. Denaturation
2. Ligation
3. Annealing
4. Extension
Which of the following enzymes are used to join bits of DNA:
1. Ligase
2. Primase
3. DNA polymerase
4. Endonuclease
Genetic engineering involves:
1. Use of restriction endonuclease on bacterial DNA and formation of new traits
2. Use of Ligase for cutting DNA
3. Developing instruments
4. Use of statistic in genetics
| 1. | Techniques of using live organisms only |
| 2. | Techniques of using enzymes only to produce products and processes useful to humans |
| 3. | The integration of natural science and organisms, cells, parts there of and molecular analogues for product and services |
| 4. | Techniques which include only synthesising a gene and using it |
A and B in the pBR 322, shown in the diagram given below, respectively represent recognition sequences of :
| 1. | BamH I and Sma I | 2. | Hind II and Sma I |
| 3. | BamH I and SaI I | 4. | SaI I and Hind II |
Recombinant DNA technology involves several steps in which initial step is of isolation of the DNA. Which enzymes are used in the process for the breakdown of fungal cell, plant cell and bacterial cell respectively?
1. Lysozyme, lipases, trypsin
2. Chitinase, cellulase, lysozyme
3. Chitinase, cellulase, trypsin
4. Trypsin, lipases, cellulase
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of:
| 1. | Insertional inactivation of alpha-galactosidase in non-recombinant bacteria |
| 2. | Insertional inactivation of beta-galactosidase in recombinant bacteria |
| 3. | Inactivation of galactase enzyme in recombinant bacteria |
| 4. | Recombinant bacteria containing beta-galactosidase |
The DNA molecule to which the gene of interest is integrated for cloning is called:
| 1. | Vector | 2. | Template |
| 3. | Carrier | 4. | Transformer |