Q. No.DNA precipitation out of a mixture of biomolecules can be achieved by treatment with:
1.
Chilled chloroform
2.
isopropanal
3.
Chilled ethanol
4.
Methanol at room temperature
The two antibiotic resistance genes on vector pBR 322 are for:
| 1. | Ampicillin and Tetracycline |
| 2. | Ampicillin and Chloramphenicol |
| 3. | Chloramphenicol and Tetracycline |
| 4. | Tetracycline and Kanamycin |
Match the following enzymes with their functions:
|
Column-I |
Column-II |
||
|
(a) |
Restriction endonuclease |
(i) |
joins the DNA fragments |
|
(b) |
Exonuclease |
(ii) |
extends primers on genomic DNA template |
|
(c) |
DNA ligase |
(iii) |
cuts DNA at specific position |
|
(d) |
Tag polymerase |
(iv) |
removes nucleotides from the ends of DNA |
Select the correct option from the following:
1. (a) - (iii), (b)- (i), (c)- (iv), (d)-(ii)
2. (a) - (iii), (b)- (iv), (c)- (i), (d)-(ii)
3. (a) - (iv), (b)- (iii), (c)- (i), (d)-(ii)
4. (a) - (ii), (b)- (iv), (c)- (i), (d)-(iii)
The enzyme used to integrate alien gene with suitable vector DNA is
1. Helicase
2. Alkaline Phosphatase
3. DNA ligase
4. Restriction endonuclease
Plant cells are bombarded with high-velocity microparticles of X coated with Y in a method called Z. Choose the option that fills the blanks correctly.
| X | Y | Z | |
| 1. | Gold or tungsten | DNA | Biolistics |
| 2. | Platinum and gold | RNA | Biolistics |
| 3. | Gold or silver | RNA | Gene gun |
| 4. | Platinum or silver | RNA | Gene gun |
In genetic engineering, the antibiotic resistance gene are used
| 1. | As selectable markers |
| 2. | To select healthy vectors |
| 3. | As sequences from where the replication starts |
| 4. | To keep culture free of infection |
Elution is a method applied for
| 1. | Making the matrix during gel electrophoresis |
| 2. | Staining the bands of DNA after electrophoresis |
| 3. | Cutting out the pieces of agarose gel and extraction of DNA from gel pieces |
| 4. | Joining the specific DNA with the cloning vector |
Which of the following processes cannot be considered a part of biotechnology?
| 1. | Genetic engineering |
| 2. | Manipulation of defective gene |
| 3. | Traditional hybridisation |
| 4. | Developing a DNA vaccine |
Mark the first isolated restriction endonuclease enzyme
1. EcoR I
2. Bam HI
3. Hind II
4. EcoR II
The processes which includes separation and purification of products are collectively called as
| 1. | Upstreaming process |
| 2. | Downstreaming process |
| 3. | Quality control testing |
| 4. | Clinical trial test |
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