Q. No.Mark the incorrect statement
1. Bam HI restriction site is present in tetracycline resistant part of pBR-322
2. Separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide
3. Agrobacterium tumefaciens is considered as natural genetic engineer
4. In the gel electrophoresis DNA fragments resolve according to base sequence
2. Separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide
3. Agrobacterium tumefaciens is considered as natural genetic engineer
4. In the gel electrophoresis DNA fragments resolve according to base sequence
A plasmid
| 1. | Cannot replicate |
| 2. | Is autonomously replicating circular extrachromosomal DNA |
| 3. | Shows dependent assortment |
| 4. | Is able to integrate with the host chromosome |
In-gel electrophoresis, the differential mobility of DNA depends on_______
| 1. | Helical nature of DNA |
| 2. | Double-stranded nature of DNA |
| 3. | Charge and size of DNA |
| 4. | Hydrogen bonding between bases |
The figure below is a diagrammatic representation of the E.coli vector pBR322, which one of the given option includes wrongly labelled components with respect to site of restriction endonuclease enzymes?
1. BamH I and Pst I
2. Pst I and Pvu I
3. Pvu I and Pvu II
4. EcoR I and Sal I
The processes which includes separation and purification of products are collectively called as
| 1. | Upstreaming process |
| 2. | Downstreaming process |
| 3. | Quality control testing |
| 4. | Clinical trial test |
Mark the first isolated restriction endonuclease enzyme
1. EcoR I
2. Bam HI
3. Hind II
4. EcoR II
Which of the following processes cannot be considered a part of biotechnology?
| 1. | Genetic engineering |
| 2. | Manipulation of defective gene |
| 3. | Traditional hybridisation |
| 4. | Developing a DNA vaccine |
Elution is a method applied for
| 1. | Making the matrix during gel electrophoresis |
| 2. | Staining the bands of DNA after electrophoresis |
| 3. | Cutting out the pieces of agarose gel and extraction of DNA from gel pieces |
| 4. | Joining the specific DNA with the cloning vector |
In genetic engineering, the antibiotic resistance gene are used
| 1. | As selectable markers |
| 2. | To select healthy vectors |
| 3. | As sequences from where the replication starts |
| 4. | To keep culture free of infection |
‘Competence’ for transformation can be induced in certain bacteria if they are treated with:
| 1. | Specific concentration of divalent cation like calcium |
| 2. | Polyethylene glycol |
| 3. | Cellulase to create protoplast |
| 4. | Colchicine |
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