Q. No.Which of the following is not correct for the organism and its cell wall degrading enzyme?
1.
bacteria - lysozyme
2.
plant cells - cellulase
3.
algae - methylase
4.
fungi - chitinase
A gene whose expression helps to identify transformed cell is known as:
1. Selectable marker
2. Vector
3. Plasmid
4. Structural gene
Two microbes found to be very useful in gene engineering are
1. Escherichia coli and Agrobacterium tumefaciens.
2. Vibrio cholerae and a tailed bacteriophage.
3. Diplococcus sp. And Pseudomonas sp.
4. Crown gall bacterium and Caenorphaditiselegans.
Which one of the following is used as a vector for cloning genes into higher organisms?
1. Retrovirus
2. Baculovirus
3. Salmonella typhimurium
4. Rhizopusnigricans
The introduction of tDNA into plants involves:
| 1. | infection of the plant by Agrobacterium tumefaciens |
| 2. | Altering the pH of the soil, then heat-shocking the plants |
| 3. | Exposing the plants to cold for a brief period |
| 4. | Allowing the plant roots to stand in water |
If the required gene has been isolated by shotgun approach, care must be taken to:
| 1. | cut the plasmid with a different restriction enzyme than that used to obtain the donor gene |
| 2. | cut the plasmid with the same restriction enzyme that was used to obtain the donor gene |
| 3. | avoid cutting the plasmid |
| 4. | cut the plasmid with two different restriction enzymes |
What is the sequence of steps in PCR?
| 1. | Amplification, insertion, polymerisation |
| 2. | Denaturation, annealing, chain elongation |
| 3. | Annealing, chain termination, denaturation |
| 4. | Annealing, amplification, polymerisations |
Complete the analogy:
Molecular scissor : Restriction endonuclease : : Molecular glue : X
| 1. | Alkaline phosphatase |
| 2. | Acid phosphatase |
| 3. | Taq polymerase |
| 4. | DNA ligase |
Gene cloning occurs:
| 1. | when a phage transfers bacterial DNA from one bacterium to another. |
| 2. | when a bacterium takes up DNA from the surrounding fluid. |
| 3. | when a bacterium carrying a recombinant plasmid reproduces, thus allowing for the production of multiple copies of the recombinant plasmid. |
| 4. | when DNA is produced from an RNA template. |
© 2026 GoodEd Technologies Pvt. Ltd.