Function of Nucleases:
 

1.	Break the polynucleotide chain by breaking each terminal nucleotide
2.	Breaks phosphodiester bond
3.	Breaks peptide bonds
4.	Breaks ester bonds

Hybridoma is:
 

The bacteria generally used for genetic engineering is:

1. Agrobacterium

2. Bacillus

3. Pseudomonas

4. Clostridium

A giant rat is formed in the laboratory, what is the reason:

1. Gene mutation

2. Gene synthesis

3. Gene manipulation

4. Gene replication

Plasmid has been used as a vector because:
 

In bacteria, the plasmid is:

1. Extrachromosomal material

2. Main DNA

3. Non-functional DNA

4. Repetitive gene

Which of the following enzymes are used to join bits of DNA:

1. Ligase

2. Primase

3. DNA polymerase

4. Endonuclease

Manipulation of DNA in genetic engineering became possible due to the discovery of:

1. Restriction endonuclease

2. DNA ligase

3. Transcriptase

4. Primase

Which one of the following bacteria has found extensive use in genetic engineering work in plants?
1. Clostridium septicum
2. Xanthomonas citri
3. Bacillus coagulens
4. Agrobacterium tumefaciens

What is true for a plasmid?
1. Plasmids are widely used in gene transfer
2. These are found in a virus
3. A plasmid contains genes for vital activities
4. This is the main part of the chromosome