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Q. No.Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:
1.
6 bp
2.
4 bp
3.
10 bp
4.
8 bp
Subtopic: Restriction Enzymes - Main Enzymes | Restriction Enzymes: Historical Background |
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The " Ti plasmid " of Agrobacterium tumefaciens stands for
| 1. | Tumor independent plasmid |
| 2. | Tumor inducing plasmid |
| 3. | Temperature independent plasmid |
| 4. | Tumour inhibiting plasmid |
Subtopic: Tools: Vectors: I |
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Which of the following statements is incorrect?
| 1. | Most commonly used bio-reactors are of stirring type. |
| 2. | Bio-reactors are used to produce small scale bacterial cultures. |
| 3. | Bio-reactors have an agitator system, an oxygen delivery system and foam control system. |
| 4. | A bio-reactor provides optimal growth conditions for achieving the desired product. |
Subtopic: Bioreactors/Downstream Processing |
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The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of 'X' and 'Y' genes :
| 1. | The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of Plasmid. |
| 2. | The gene 'X' is 'for protein involved in replication of Plasmid and 'Y' for resistance to antibiotics. |
| 3. | Gene 'X' is responsible for recognition sites and 'Y' is responsible for antibiotic resistance. |
| 4. | The gene 'X' is responsible for resistance to antibiotics and 'Y' for protein involved in the replication of Plasmid. |
Subtopic: Cloning Vector |
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Which of the following is not a selectable marker of cloning vectors?
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
Subtopic: Cloning Vector |
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Match List-I with List-II
Choose the correct answer from the options given below:
1. A-III, B-IV, C-I , D-II
2. A-III, B-I, C-IV, D-II
3. A-IV, B-III, C-II , D-I
4. A-II, B-IV, C-I, D-III
| List-I | List-II | ||
| A. | Ampicillin | I. | Restriction sites |
| B. | Ori | II. | Blue/White screening |
| C. | Multiple cloning sites | III. | Selectable marker |
| D. | \(\beta\) galactosidase gene | IV. | Sequence from where replication starts |
1. A-III, B-IV, C-I , D-II
2. A-III, B-I, C-IV, D-II
3. A-IV, B-III, C-II , D-I
4. A-II, B-IV, C-I, D-III
Subtopic: Cloning Vector |
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Given below are two statements:
In light of the above statements, choose the correct answer from the options given below:
1. Both Statement I and Statement II are True
2. Both Statement I and Statement II are False
3. Statement I is True but Statement II is False
4. Statement I is False but Statement II is True
| Statement I: | Restriction Endonuclease finds its specific recognition sequence and binds to the DNA. |
| Statement II: | Restriction Endonuclease cuts each of the two strands of the double helix at specific points in their sugar phosphate backbones. |
1. Both Statement I and Statement II are True
2. Both Statement I and Statement II are False
3. Statement I is True but Statement II is False
4. Statement I is False but Statement II is True
Subtopic: Restriction Enzymes - Main Enzymes |
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What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism?
Choose the correct answer from the options given below:
| A: | The piece of DNA would be able to multiply itself independently in the progeny cells of the organism. |
| B: | It may get integrated into the genome of the recipient. |
| C: | It may multiply and be inherited along with the host DNA. |
| D: | The alien piece of DNA is not an integral part of chromosome. |
| E: | It shows ability to replicate. |
| 1. | D and E only | 2. | B and C only |
| 3. | A and E only | 4. | A and B only |
Subtopic: Principles of Biotechnology |
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In a chromosome, there is a specific DNA sequence, responsible for initiating replication. It is called as:
| 1. | recognition sequence | 2. | cloning site |
| 3. | restriction site | 4. | ori site |
Subtopic: Principles of Biotechnology |
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Identify the incorrect statement related to electrophoresis:
| 1. | Separated DNA fragments can be directly seen under UV radiation |
| 2. | Separated DNA can be extracted from gel piece. |
| 3. | Fragments of DNA move toward anode. |
| 4. | Sieving effect of agarose gel helps in separation of DNA fragments. |
Subtopic: Separation and Isolation of DNA fragments |
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