Select Chapter Topics:
Q. No.Which of the following can replicate within bacterial cells independent of the control of chromosomal DNA?
I:
Plasmid
II:
Phage DNA
1. Only I
2. Only II
3. Both I and II
4. Neither I nor II
Subtopic: Â Cloning Vector |
 55%
Level 3: 35%-60%
Please attempt this question first.
Hints
Please attempt this question first.
A plasmid has two genes providing resistance to antibiotics A and B respectively. A foreign DNA has been inserted within the gene for resistance to antibiotic A:
1. Only I and II are correct
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
| I. | Non-Transformants will not survive in a medium containing antibiotic A and in a medium containing antibiotic B. |
| II. | Recombinant transformants will survive in a medium containing antibiotic B but not in a medium containing antibiotic A. |
| III. | Non-recombinant transformants will survive in a medium containing antibiotic A and in a medium containing antibiotic B. |
2. Only I and III are correct
3. Only II and III are correct
4. I, II and III are correct
Subtopic: Â Tools: Vectors: I |
Level 3: 35%-60%
Please attempt this question first.
Hints
Please attempt this question first.
Consider the two given statements:
| Assertion (A): | Foreign and vector DNA must be cleaved with the same restriction enzyme when making recombinant DNA. |
| Reason (R): | Each restriction enzyme cuts DNA at a specific cleavage site. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | (A) is True; (R) is False |
| 3. | (A) is False; (R) is True |
| 4. | Both (A) and (R) are True but (R) does not correctly explain (A). |
Subtopic: Â Restriction Enzymes - Main Enzymes |
Level 3: 35%-60%
Please attempt this question first.
Hints
Please attempt this question first.
Consider the given two statements:
| Assertion (A): | Restriction enzymes are found in bacteria and provide a defense mechanism against invading viruses. |
| Reason (R): | They interact with membranes of the host bacterial cells and remove all the receptor sites by the viruses. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True; (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Â Restriction Enzymes - Main Enzymes |
 55%
Level 3: 35%-60%
Please attempt this question first.
Hints
Please attempt this question first.
Consider the given two statements:
| Assertion(A): | Any piece of DNA when linked to Ori sequence of a suitable vector can be made to replicate within the host cells. |
| Reason (R): | Ori sequence is responsible for controlling the copy number of the linked DNA. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True; (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Â Cloning Vector |
Level 3: 35%-60%
Please attempt this question first.
Hints
Please attempt this question first.
| Assertion (A): | Synthetic oligonucleotide polymers are used during annealing in a PCR. |
| Reason (R): | The primers bind to the double stranded DNA at their complementary regions. |
| 1. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are True and (R) is not the correct explanation of (A). |
| 3. | (A) is True but (R) is False. |
| 4. | (A) is False but (R) is True. |
Subtopic: Â Polymerase Chain Reaction: PCR |
Level 3: 35%-60%
Please attempt this question first.
Hints
Please attempt this question first.
The process by which a virus transfers genetic material from one bacterium to another is called:
| 1. | Transduction | 2. | Conjugation |
| 3. | Transformation | 4. | Translation |
Subtopic: Â Tools |
 51%
Level 3: 35%-60%
Please attempt this question first.
Hints
Please attempt this question first.
'Restriction' in Restriction enzyme refers to:
| 1. | Cleaving of phosphodiester bond in DNA by the enzyme |
| 2. | Cutting of DNA at specific position only |
| 3. | Prevention of the multiplication of bacteriophage by the host bacteria |
| 4. | All of the above |
Subtopic: Â Restriction Enzymes - Main Enzymes |
Level 3: 35%-60%
Please attempt this question first.
Hints
Please attempt this question first.
Consider the given two statements:
| Assertion (A): | Plasmid DNA is used to monitor bacterial transformation with foreign DNA. |
| Reason (R): | Plasmid is extrachromosomal DNA in some bacteria. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A) |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A) |
| 3. | (A) is True but (R) is False |
| 4. | (A) is False but (R) is True |
Subtopic: Â Cloning Vector |
Level 3: 35%-60%
Please attempt this question first.
Hints
Please attempt this question first.
Select the correct match w.r.t steps involved in PCR.
| 1. | Denaturation | - | Phosphodiester bonds break |
| 2. | Annealing | - | Primers anneal at 5' end of DNA template |
| 3. | Extension | - | Taq polymerase forms hydrogen bonds between adjacent nucleotides of growing DNA strand |
| 4. | Extension | - | Thermostable DNA polymerase adds nucleotide to 3' end of primers |
Subtopic: Â Polymerase Chain Reaction: PCR |
Level 3: 35%-60%
Please attempt this question first.
Hints
Please attempt this question first.
Select Chapter Topics:
© 2026 GoodEd Technologies Pvt. Ltd.