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Q. No.Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tetR) gene in the pBR322 cloning vector.
1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI
Subtopic: Cloning Vector |
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Recombinant DNA molecule can be created normally by cutting the vector DNA and source DNA respectively with:
| 1. | Hind II, Hind II | 2. | Hind II, Alu I |
| 3. | Hind II, EcoR I | 4. | Hind II, Bam HI |
Subtopic: Tools: Enzymes: I | Tools: Enzymes: II |
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Which of the following are correct about EcoRI?
Choose the correct answer from the options given below:
| A. | Cut the DNA with blunt end |
| B. | Cut the DNA with sticky end |
| C. | Recognises a specific palindromic sequence. |
| D. | Cut the DNA between the base G and A where it encounters the DNA sequence 'GAATTC' |
| E. | Exonuclease |
| 1. | B,C,E only | 2. | A,D,E only |
| 3. | A,C,D only | 4. | B,C,D only |
Subtopic: Restriction Enzymes - Main Enzymes |
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Following are the steps involved in the process of PCR.
A. Annealing
B. Amplification (~1 billion times)
C. Denaturation
D. Treatment with Taq polymerase and deoxynucleotides
E. Extension
Choose the correct sequence of steps of PCR from the options given below :
1. C → A → D → E → B
2. A → B → E → D → C
3. A → C → E → D → B
4. D → B → E → C → A
A. Annealing
B. Amplification (~1 billion times)
C. Denaturation
D. Treatment with Taq polymerase and deoxynucleotides
E. Extension
Choose the correct sequence of steps of PCR from the options given below :
1. C → A → D → E → B
2. A → B → E → D → C
3. A → C → E → D → B
4. D → B → E → C → A
Subtopic: Polymerase Chain Reaction: PCR |
86%
Level 1: 80%+
NEET - 2024
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Identify the incorrect statement related to electrophoresis:
| 1. | Separated DNA fragments can be directly seen under UV radiation |
| 2. | Separated DNA can be extracted from gel piece. |
| 3. | Fragments of DNA move toward anode. |
| 4. | Sieving effect of agarose gel helps in separation of DNA fragments. |
Subtopic: Separation and Isolation of DNA fragments |
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In a chromosome, there is a specific DNA sequence, responsible for initiating replication. It is called as:
| 1. | recognition sequence | 2. | cloning site |
| 3. | restriction site | 4. | ori site |
Subtopic: Principles of Biotechnology |
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Given below are two statements:
In light of the above statements, choose the correct answer from the options given below:
1. Both Statement I and Statement II are True
2. Both Statement I and Statement II are False
3. Statement I is True but Statement II is False
4. Statement I is False but Statement II is True
| Statement I: | Restriction Endonuclease finds its specific recognition sequence and binds to the DNA. |
| Statement II: | Restriction Endonuclease cuts each of the two strands of the double helix at specific points in their sugar phosphate backbones. |
1. Both Statement I and Statement II are True
2. Both Statement I and Statement II are False
3. Statement I is True but Statement II is False
4. Statement I is False but Statement II is True
Subtopic: Restriction Enzymes - Main Enzymes |
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Which of the following is not a selectable marker of cloning vectors?
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
Subtopic: Cloning Vector |
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The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of 'X' and 'Y' genes :
| 1. | The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of Plasmid. |
| 2. | The gene 'X' is 'for protein involved in replication of Plasmid and 'Y' for resistance to antibiotics. |
| 3. | Gene 'X' is responsible for recognition sites and 'Y' is responsible for antibiotic resistance. |
| 4. | The gene 'X' is responsible for resistance to antibiotics and 'Y' for protein involved in the replication of Plasmid. |
Subtopic: Cloning Vector |
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Which of the following statements is incorrect?
| 1. | Most commonly used bio-reactors are of stirring type. |
| 2. | Bio-reactors are used to produce small scale bacterial cultures. |
| 3. | Bio-reactors have an agitator system, an oxygen delivery system and foam control system. |
| 4. | A bio-reactor provides optimal growth conditions for achieving the desired product. |
Subtopic: Bioreactors/Downstream Processing |
87%
Level 1: 80%+
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