Q. No.The DNA molecule to which the gene of interest is integrated for cloning is called:
1.
Transformer
2.
Vector
3.
Template
4.
Carrier
The DNA fragments separated on an agarose gel can be visualized after staining with:
| 1. | Acetocarmine | 2. | Aniline blue |
| 3. | Ethidium bromide | 4. | Bromophenol blue |
The process of separation and purification of expressed protein before marketing is called:
1. Downstream processing
2. Bioprocessing
3. Post-product processing
4. Upstream processing
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using?
| 1. | Eco Rl | 2. | taq polymerase |
| 3. | polymerase lll | 4. | ligase |
Which of the following restriction enzymes produces blunt ends?
1. Sal l
2. Eco RV
3. Xho
4. Hind lll
DNA fragments are:
1. Negatively charged
2. Neutral
3. Either positively charged depending on their size
4. Positively charged
A gene whose expression helps to identify transformed cell is known as:
1. Vector
2. Plasmid
3. Structural gene
4. Selectable marker
What is the criterion for the movement of DNA fragments on agarose gel during gel electrophoresis?
| 1. | The smaller the fragment size, the farther it moves |
| 2. | Positively charged fragments move to farther end |
| 3. | Negatively charged fragments do not move |
| 4. | The larger the fragment size, the farther it moves |
2. DNase I
3. RNase
4. Hind II
1. Circular structure
2. Transferable
3. Single-stranded
4. Independent replication
© 2026 GoodEd Technologies Pvt. Ltd.