Gel electrophoresis is used for

1. cutting of DNA into fragments

2. separation of DNA fragments according to their size

3. construction of recombinant DNA by joining with cloning vectors

4. isolation of DNA molecule

Which of these are most widely used in genetic engineering?

1. Plastid

2. Plasmid

3. Mitochondrion

4. Ribosome

Introduction of  foreign genes for improving phenotype is

1. biotechnology 

2. vernalization

3. tissue culture

4. genetic engineering

A tumor inducing plasmid widely used in the production of transgenic plants is that of

1. Escherichia coli

2. Bacillus thuringiensis

3. Staphylococcus aureus

4. Agrobacterium tumefaciens.

What is the source of EcoRI?

1. Escherichia coli R 1
2. Escherichia coli R I 13
3. Escherichia coli R Y 13
4. Escherichia coli R X 13

Which of the following is a cloning vector?

How are transformants selected from nontransformants?

Which of the following is not a characteristic of pBR322 vector? 

1. It is the first artificial cloning vector constructed in 1977 by Boliver and Rodriguez.

2. It is the most widely used, versatile and easily manipulated vector.

3. It has two antibiotic resistance genes, tet^R and amp^R.

4. It does not have restriction site for Sal I.

Plasmid vector in DNA recombinant technology means

Biolistic (gene gun) is suitable for