| A. | Substrate enzyme complex formation. |
| B. | Free enzyme ready to bind with another substrate. |
| C. | Release of products. |
| D. | Chemical bonds of the substrate broken. |
| E. | Substrate binding to active site. |
| 1. | Endothermic reaction with energy A in the presence of enzyme and B in the absence of enzyme |
| 2. | Exothermic reaction with energy A in the presence of enzyme and B in the absence of enzyme |
| 3. | Endothermic reaction with energy A in the absence of enzyme and B in the presence of enzyme |
| 4. | Exothermic reaction with energy A in the absence of enzyme and B in the presence of enzyme |
Transition state structure of the substrate formed during an enzymatic reaction is:
| 1. | permanent but unstable |
| 2. | transient and unstable |
| 3. | permanent and stable |
| 4. | transient but stable |