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The radius of the double helix of B-DNA [Watson and Crick model] is approximately:

1.1.0 nm2.2.0 nm
3.0.34 nm4.3.4 nm

Subtopic:  DNA Double Helix |
Level 3: 35%-60%

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An RNA primer is essential for DNA synthesis carried out by DNA polymerase III. This is because, to synthesize DNA, DNA polymerase III requires:

1.  A free 3’ – PO4 group
2.  A free 5’ – PO4 group
3.  A free 3’ – OH group
4.  A free 5’ – OH group

Subtopic:  DNA Replication |
 67%
Level 2: 60%+

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Which of the following possible modes of replication was eliminated by Meselson and Stahl based on the finding after one generation of replication of the bacterium?

1. Dispersive 2. Semi-conservative
3. Conservative 4. Both 1 and 3

Subtopic:  Search for Genetic Material |
Level 4: Below 35%

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In eukaryotes, RNA polymerase III transcribes:

1. hnRNA, snRNAs and 5.8S RNA
2. tRNA, 5.8S RNA and snRNAs
3. tRNA, 5SrRNA and snRNAs
4. rRNAs – 28S, 18S and 5S

Subtopic:  Transcription: I |
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Level 2: 60%+

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Identify the incorrect statement:

1. The DNA of the bacteriophage  ϕ X 174 has 5386 nucleotides.
2. Bacteriophage lambda DNA has 48502 base pairs.
3. The DNA of Escherichia coli has 4.6 X 103 base pairs.
4. Haploid content of human DNA is 3.3 X 109 base pairs.
Subtopic:  The DNA |
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Level 1: 80%+

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In a DNA strand, two nucleotides are linked through a ___________ linkage to form a dinucleotide.

1. 3’ – 5’ phosphodiester bond

2. 5’ – 3’ phosphodiester bond

3. 1’ N-glycosidic bond

4. Two or three hydrogen bonds

Subtopic:  DNA Double Helix |
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Level 2: 60%+

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Replication in prokaryotes differs from replication in eukaryotes for which of the following reasons?

1. Prokaryotic chromosomes have histones, whereas eukaryotic chromosomes do not.
2. Prokaryotic chromosomes have a single origin of replication, whereas eukaryotic chromosomes have many.
3. The rate of elongation during DNA replication is slower in prokaryotes than in eukaryotes.
4. Prokaryotes produce Okazaki fragments during DNA replication, but eukaryotes do not.
Subtopic:  DNA Replication: I |
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Level 2: 60%+

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Consider the following two statements:

I: During transcription, the template strand is read in a 3'-to-5' direction.
II: During transcription, an RNA is transcribed in the 5'-to-3' direction.

The correct statements is/are:

1. I only 2. II Only
3. I and II 4. None
Subtopic:  Transcription: I |
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Level 2: 60%+

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Identify the correct statements from the given statements and choose the correct option:

I: Both prokaryotic and eukaryotic mRNAs have a 5' cap.
II: Only prokaryotic mRNAs are polyadenylated at the 3' end.
III: In prokaryotes, transcription is coupled to translation.
IV: In eukaryotes, RNA splicing occurs after the mRNA is transported into the cytoplasm.
V: RNA splicing requires the formation of a spliceosome.
VI: Both prokaryotic and eukaryotic mRNAs are synthesized by RNA polymerase.

1. III, V, and VI only
2. II, III, V, and VI only
3. I, III, V, and VI only
4. I, III, IV, V, and VI only
Subtopic:  Transcription: I | Translation | Translation Mechanism |
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Level 2: 60%+

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Which radioactive isotope was used in Meselson and Stahl experiment to prove the semi-conservative mode of DNA replication?
1. 35S 2. 32P
3. 15N 4. None
Subtopic:  Search for Genetic Material |
 50%
Level 3: 35%-60%

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