In a DNA gel the fragment of 2kb and 3kb will be

1. 2kb nearer to cathode while 3kb nearer to anode

2. 2kb closer to wells while 3kb towards the opposite ends

3. 2kb closer to opposite end of gel while 3kb closer to well

4. 2kb away from cathode while 3kb towards anode

rop segment codes for pBR322?

1. Resistance protein

2. Protein for replication

3. Proteins for translation

4. Cloning site

The synthesis of DNA in PCR will be in

1. 5’-3’

2. 3’-5’

3. It won't be depending on direction

4. For one strand 5’-3’ in another opposite to it

The primers designed according to (Answer according to very basic PCR)

1. Terminal ends of vector

2. Terminal ends of gene of interest

3. Random primers

4. According to direction of PCR

Continuous culture leads to

1. Smaller biomass

2. Higher yields of desired proteins

3. Both A and B

4. Higher yields of recombinant DNA

The Agitation system of bioreactor has

1. Flat bladed impeller

2. Sharp impellers

3. Foam breaker

4. Steamer

Increased surface area for oxygen transfer is a feature found specifically in

1. Simple stirred tank bioreactor

2. Sparged stirred-tank bioreactor

3. All stirred tank bioreactors

4. All bioreactors have this property

Today we know more than …….. restriction enzymes that have been isolated from over……..strains

1. 700; 350

2. 900; 230

3. 600; 270

4. 550; 210

Restriction enzymes are synthesized by:

1. Bacteria only

2. Yeast and bacteria only

3. Eukaryotic cells only

4. All kinds of cells

Following are vectorless gene transfer except one. Mark the except one

(1) Micro injection

(2) Electroporation

(3) Cosmid

(4) Biolistic