The correct order of steps in Polymerase Chain Reaction (PCR) is:

1. Extension, Denaturation, Annealing
2. Annealing, Extension, Denaturation
3. Denaturation, Extension, Annealing
4. Denaturation, Annealing, Extension

Subtopic:  Polymerase Chain Reaction: PCR |
 84%
From NCERT
NEET - 2018
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DNA fragments are:

1. Negatively charged

2. Neutral

3. Either positively charged depending on their size

4. Positively charged

Subtopic:  Separation and Isolation of DNA fragments |
 93%
From NCERT
NEET - 2017
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Which of the following is a restriction endonuclease?
1. Protease
2. DNase I
3. RNase
4. Hind II
Subtopic:  Tools: Enzymes: II | Tools |
 95%
From NCERT
NEET - 2016
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Which of the following is not a feature of the plasmids?
1. Circular structure
2. Transferable
3. Single-stranded
4. Independent replication
Subtopic:  Tools: Vectors: I |
 76%
From NCERT
NEET - 2016
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The introduction of t-DNA into plants involves?

1. Infection of the plant by Agrobacterium tumefaciens

2. Altering the pH of the soil, heat-shocking the plants

3. Exposing the plants to Cold for a brief period

4. Allowing the plant roots to stand in water

Subtopic:  Tools: Vectors: II | Tools |
 91%
From NCERT
NEET - 2015
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Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?

1. Plant cells- cellulose

2. Algae - Methylase

3. Fungi - Chitinase

4. Bacteria - Lysozyme

Subtopic:  Obtaining Copy of Gene from Donor DNA |
 86%
From NCERT
AIPMT - 2013
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Which one is a true statement regarding DNA polymerase used in PCR?

1. It is used to ligate introduces DNA in recipient cells

2. It serves as a selectable marker

3. It is isolated from a virus

4. It remains active at high temperatures

Subtopic:  Polymerase Chain Reaction: PCR |
 77%
From NCERT
AIPMT - 2012
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Agarose extracted from seaweeds finds use in:
1.  tissue culture
2.  PCR
3.  gel electrophoresis
4.  spectrophotometry
Subtopic:  Separation and Isolation of DNA fragments |
 95%
From NCERT
AIPMT - 2011
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Restriction endonucleases are enzymes which:

1. make cuts at specific positions within the DNA molecule
2. recognize a specific nucleotide sequence for binding
of DNA ligase
3. restrict the action of the enzyme DNA polymerase
4. remove nucleotides from the ends of the DNA molecule 

Subtopic:  Restriction Enzymes - Main Enzymes |
 92%
From NCERT
AIPMT - 2010
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In attempts to confer special characteristics upon plants, genetic engineers find Agrobacterium tumefaciens to be an effective vector for use with

1. corn

2. rice

3. wheat

4. soy beans

Subtopic:  Tools: Vectors: II | Tools |
 58%
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