The source of taq polymerase used in PCR is a
(1) thermophilic fungus
(2) mesophilic fungus
(3) thermophilic bacterium
(4) halophilic bacterium
The process of separation and purification of expressed protein before marketing is called
(1) upstream processing
(2) downstream processing
(3) bioprocessing
(4) postproduction processing
The product has to be subjected through a series of processes before it is ready for marketing as a finished product called down stream processing. It includes
1. Separation
2. Purification
3. Quality control
4. Both 1 & 2
What is true about the DNA polymerase used in PCR?
(1) Isolated from a virus
(2) Stable at high temperatures
(3) Used as selectable marker
(4) Has methylated bases
Which of the following equipment is essentially required for growing microbes on a large scale for the industrial production of enzymes?
1. | Bioreactor | 2. | BOD incubator |
3. | Sludge digester | 4. | Industrial oven |
Taq polymerase is used in the polymerase chain reaction because:
1. It replicates DNA faster than other enzymes
2. It is the only enzyme that can replicate DNA invitro
3. It does not require an RNA primer to function
4. It is thermostable
Separation and purification by filtration, centrifugation of desired compound produced in bioreactor is a part of
1. Downstream processing only
2. Scaling up and downstream processing
3. Upstream processing
4. Screening for recombinants and downstream processing
During gel electrophoresis for separation of DNA fragment:
1. Smallest fragment will move to the farthest point towards cathode
2. Smallest fragment will move to the farthest point towards anode
3. Largest fragment will move to the farthest point towards cathode
4. Largest fragment will move to the farthesr point towards anode
Stirred-tank bioreactors have been designed for:
1. purification of product
2. addition of preservatives to the product
3. availability of oxygen throughout the process
4. ensuring anaerobic conditions in the culture vessel
Which of the following is not a component of downstream processing?
1. Separation
2. Purification
3. Preservation
4. Expression